Protein tyrosine phosphatase-1C is rapidly phosphorylated in tyrosine in macrophages in response to colony

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Updated: Aug 9, 2026

Oligopeptide Competition Assay for Phosphorylation Site Determination
09:16

Oligopeptide Competition Assay for Phosphorylation Site Determination

Published on: May 18, 2017

Y G Yeung1, K L Berg, F J Pixley

  • 1Department of Developmental Biology and Cancer, Albert Einstein College of Medicine, Bronx, New York 10461.

The Journal of Biological Chemistry
|November 25, 1992
PubMed

Insights

Colony stimulating factor-1 rapidly induces tyrosine phosphorylation of a 64-kDa protein in macrophages. This protein was identified as src homology 2 domain-containing protein tyrosine phosphatase-1C (PTP-1C), suggesting its role in early growth factor signaling.

Area of Science:

  • Cellular signaling
  • Molecular biology
  • Immunology

Background:

  • Macrophages respond to colony stimulating factor-1 (CSF-1) through complex signaling pathways.
  • Tyrosine phosphorylation is a key regulatory mechanism in cellular signal transduction.

Purpose of the Study:

  • To identify the approximately 64-kDa cytoplasmic protein rapidly phosphorylated in tyrosine upon CSF-1 stimulation.
  • To investigate the role of this protein in early growth factor signal transduction.

Main Methods:

  • BAC1.2F5 macrophages were stimulated with CSF-1.
  • Cytosolic fractions were analyzed using size exclusion chromatography and reverse-phase high-performance liquid chromatography (RP-HPLC).
  • Tryptic peptides were sequenced, and results were confirmed by Western blotting using an anti-PTP-1C antibody.

Main Results:

  • A 64-kDa protein was identified as src homology 2 domain-containing protein tyrosine phosphatase-1C (PTP-1C).
  • CSF-1 stimulation led to rapid tyrosine phosphorylation of PTP-1C.
  • Seven unique peptide sequences from the stimulated fraction matched the PTP-1C sequence.

Conclusions:

  • PTP-1C is rapidly tyrosine phosphorylated in response to CSF-1 in macrophages.
  • This phosphorylation suggests PTP-1C plays a role in the early stages of CSF-1-induced signal transduction.

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