Related Experiment Video
Updated: Aug 12, 2026
Detection of Human Leukocyte Antigen Biomarkers in Breast Cancer Utilizing Label-free Biosensor Technology
Published on: March 24, 2015
A monoclonal antibody to common acute lymphoblastic leukemia antigen (CALLA) and its expression on several human
1Department of Pathology, Seoul National University College of Medicine, Korea.
Insights
A new murine monoclonal antibody, SHB-10, recognizes the common acute lymphoblastic leukemia antigen (CALLA). SHB-10 shows similar binding to anti-CD10 antibodies but may detect subtle differences in antigen expression on certain cell types.
Area of Science:
- Immunology
- Oncology
- Biochemistry
Background:
- The common acute lymphoblastic leukemia antigen (CALLA), also known as CD10, is a significant biomarker in lymphoid malignancies.
- Characterizing novel antibodies targeting CALLA is crucial for diagnostic and therapeutic advancements in leukemia and other cancers.
Purpose of the Study:
- To describe a newly developed murine monoclonal antibody, designated SHB-10.
- To evaluate the specificity and binding characteristics of SHB-10 against the CALLA.
- To investigate the expression profile of the antigen recognized by SHB-10 on various cell lines.
Main Methods:
- Production and characterization of a murine monoclonal antibody (SHB-10).
- Indirect flow cytometry using lymphoid malignant cell lines and peripheral lymphocytes from acute lymphoblastic leukemia (ALL) patients.
- Blocking assays to assess epitope specificity by comparing SHB-10 binding with conventional anti-CD10 antibodies.
- Analysis of antigen distribution on neuroectodermal tumor and lymphoid malignancy cell lines.
Main Results:
- SHB-10 detects an antigen with an approximate molecular weight of 105 kDa.
- Flow cytometric analysis indicates SHB-10 exhibits high similarity to conventional anti-CD10 antibodies.
- SHB-10 binding to Daudi cells and ALL patient lymphocytes is blocked by anti-CD10, confirming CALLA recognition.
- A slight difference in cell surface expression of the SHB-10-detected antigen was observed compared to conventional anti-CD10 on tested cell lines.
Conclusions:
- The monoclonal antibody SHB-10 is a valuable tool for detecting the common acute lymphoblastic leukemia antigen (CALLA).
- SHB-10 demonstrates comparable reactivity to established anti-CD10 antibodies.
- Further biochemical analysis is warranted to fully characterize the antigen and elucidate potential differences in its cell surface expression compared to conventional anti-CD10.
Abstract:
We describe a newly-made murine monoclonal antibody to the common acute lymphoblastic leukemia antigen (CALLA), named SHB-10. The antigen detected by SHB-10 has a molecular weight of about 105 kDa. This antibody is very similar to that of conventional anti-CD10 Ab on indirect flowcytometric analysis using lymphoid malignant cell lines and peripheral lymphocytes of acute lymphoblastic leukemia (ALL) patients. The binding of anti-CD10 to Daudi cell and peripheral lymphocytes of ALL patients is blocked by SHB-10. Thus this monoclonal antibody is thought to detect the CALLA. The distribution of antigen detected by SHB-10 on several cell lines of neuroectodermal tumor and lymphoid malignancy was analysed and a slight difference in their cell surface expression is observed when compared with that by conventional anti-CD10. Further biochemical analysis is now under way for a better characterization of this antigen.
More Related Videos
Related Concept Videos
01:15Differentiation of Common Myeloid Progenitor Cells
01:31Hybridoma Technology
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...