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Characterization of Fc epsilon RI gamma in human natural killer cells

P Moingeon1, J L Lucich, E L Reinherz

  • 1Laboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, MA.

International Immunology
|August 1, 1992
PubMed

Insights

The Fc epsilon RI gamma chain is part of IgG receptors on NK cells and IgE receptors on basophils. Its presence doesn't directly correlate with NK cell cytotoxicity, indicating diverse roles in the immune system.

Area of Science:

  • Immunology
  • Molecular Biology

Background:

  • The Fc epsilon RI gamma chain is a component of high-affinity IgE receptors on human basophils.
  • The low-affinity IgG receptor (Fc gamma RIII) on NK cells involves CD16 and associates with Fc epsilon RI gamma.

Purpose of the Study:

  • To characterize the Fc epsilon RI gamma chain in human NK cells.
  • To investigate the association of Fc epsilon RI gamma with CD16 and its role in NK cell function.

Main Methods:

  • cDNA cloning and sequence analysis of Fc epsilon RI gamma from NK cell lines.
  • Polymerase chain reaction (PCR) analysis of Fc epsilon RI gamma gene expression in NK cell clones.

Main Results:

  • Fc epsilon RI gamma from NK cells is identical to that found in basophils.
  • Fc epsilon RI gamma expression is not directly linked to NK cell cytotoxicity, as some cytotoxic NK clones lack Fc epsilon RI gamma RNA.
  • Fc epsilon RI gamma is expressed in various hematopoietic cells as part of multimeric receptors.

Conclusions:

  • The Fc epsilon RI gamma chain plays a role in both IgE and IgG receptor complexes.
  • Fc epsilon RI gamma is not essential for NK cell-mediated cytotoxicity.
  • This molecule contributes to diverse immune functions through its involvement in different multimeric surface receptors.

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