Related Experiment Video

Updated: Aug 31, 2026

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
10:21

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Published on: February 21, 2018

GPL, a novel cytokine receptor related to GP130 and leukemia inhibitory factor receptor

Caroline Diveu1, Eric Lelièvre, David Perret

  • 1INSERM U564, CHU d'Angers, 49033 Angers, France.

The Journal of Biological Chemistry
|September 25, 2003
PubMed

Insights

A novel cytokine receptor, GP130 Like receptor (GPL), was identified with similarities to IL-6 and IL-12 receptors. GPL expression in immune cells suggests a role in Th1 immune responses and cell signaling.

Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • Cytokine receptors are crucial for cell signaling in the immune system.
  • The interleukin-6 (IL-6) and interleukin-12 (IL-12) families mediate diverse cellular responses.
  • Understanding novel cytokine receptors can elucidate new signaling pathways.

Purpose of the Study:

  • To identify and characterize a novel cytokine receptor.
  • To investigate the structure, gene organization, and expression patterns of the new receptor.
  • To explore the functional role of the novel receptor in immune cell signaling.

Main Methods:

  • Isolation and sequencing of four GPL isoforms.
  • Analysis of gene structure, localization, and homology to known receptors.
  • Detection of GPL transcripts in various tissues and cell types.
  • Functional studies using chimeric receptors to investigate signaling pathways.

Main Results:

  • A novel cytokine receptor, GP130 Like receptor (GPL), was identified with structural similarities to IL-6 and IL-12 receptors.
  • Four GPL isoforms were characterized, featuring a cytokine binding domain and intracellular Janus kinase interaction motifs.
  • GPL gene is located on chromosome 5q11.2, adjacent to the gp130 gene.
  • GPL transcripts were detected in reproductive tissues, blood cells, and the myelomonocytic lineage, with expression upregulated by interferon-gamma in monocytes and dendritic cells.
  • Chimeric receptor studies demonstrated that GPL associates with gp130 or LIF receptor intracellular domains to initiate signaling cascades.

Conclusions:

  • GPL represents a novel cytokine receptor with potential roles in reproduction and immune responses.
  • Its expression pattern and regulation by interferon-gamma suggest involvement in Th1-type immune responses.
  • GPL contributes to cell signaling pathways through its interaction with intracellular signaling molecules like Janus kinases.

More Related Videos

Identification of Functional Protein Regions Through Chimeric Protein Construction11:39

Identification of Functional Protein Regions Through Chimeric Protein Construction

Published on: January 8, 2019

A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 406:56

A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4

Published on: March 10, 2018

Related Concept Videos

Small GTPases - Ras and Rho01:24

Small GTPases - Ras and Rho

Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
G Protein-coupled Receptors01:15

G Protein-coupled Receptors

G Protein-Coupled Receptors or GPCRs are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to sensory stimuli such as light, odors, hormones, cytokines, or neurotransmitters.
GPCRs are also called heptahelical, 7TM, or serpentine receptors, and consist of seven (H1-H7) transmembrane alpha-helices that span the bilayer to form a cylindrical core. The transmembrane helices are connected by three extracellular loops and three...
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
G Protein-coupled Receptors01:27

G Protein-coupled Receptors

G-protein-coupled receptors are ligand-binding receptors that indirectly affect changes in the cell. The actual receptor is a single polypeptide that traverses the cell membrane seven times, creating intracellular and extracellular loops. The extracellular loops create a ligand-specific pocket which binds to neurotransmitters or hormones. The intracellular loops hold onto the G-protein.The G-protein, or guanine nucleotide-binding protein, is a large heterotrimeric complex. Its three subunits...
G-protein Coupled Receptors01:27

G-protein Coupled Receptors

G-protein-coupled receptors are ligand-binding receptors that indirectly affect changes in the cell. The actual receptor is a single polypeptide that traverses the cell membrane seven times, creating intracellular and extracellular loops. The extracellular loops create a ligand-specific pocket which binds to neurotransmitters or hormones. The intracellular loops hold onto the G-protein.The G-protein, or guanine nucleotide-binding protein, is a large heterotrimeric complex. Its three subunits...
IP3/DAG Signaling Pathway01:11

IP3/DAG Signaling Pathway

Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the  phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and produces two-second...