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Activation and proliferation of T lymphocyte subpopulations in patients with brucellosis

Martha Cecilia Moreno-Lafont1, Rubén López-Santiago, Vladimir Paredes-Cervantes

  • 1Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, Mexico.

Archives of Medical Research
|October 22, 2003
PubMed

Insights

This study shows that T-cells in brucellosis patients activate and proliferate in response to Brucella antigen. These findings highlight the role of CD4+ and CD8+ T cells in fighting Brucella infections.

Area of Science:

  • Immunology
  • Infectious Diseases
  • Cellular Biology

Background:

  • T-cell proliferation is key for assessing immune responses to intracellular pathogens.
  • Brucellosis, caused by Brucella, requires evaluation of cellular immunity.
  • Salt-extractable antigen from Brucella melitensis 16M (RCM-BM) was used to assess immune response.

Purpose of the Study:

  • To evaluate cellular immune response against RCM-BM antigen in brucellosis patients.
  • To assess the expression of early activation marker CD69 on T-cells.
  • To measure T-cell proliferation using nonradioactive methods.

Main Methods:

  • Flow cytometry was used to determine CD69 expression on CD4+ and CD8+ T-cells.
  • Lymphoproliferation was assessed by measuring DNA synthesis via 5-bromo-2'-deoxyuridine (BrdU) incorporation.
  • Tritiated thymidine incorporation was also used to evaluate DNA synthesis.

Main Results:

  • Thirty healthy donors and 24 brucellosis patients participated.
  • Mitogen stimulation induced CD69 expression and T-cell proliferation in all subjects.
  • Brucellosis patients showed significant CD69 expression and T-cell proliferation against RCM-BM antigen (p < 0.001).

Conclusions:

  • The methods employed are effective for evaluating immune responses to specific antigens and polyclonal stimulation.
  • Both CD4+ and CD8+ T-cells from brucellosis patients demonstrated activation and proliferation upon RCM-BM antigen exposure.
  • These results indicate that both T-cell subpopulations are crucial in the immune response against Brucella infections.
Abstract

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