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Updated: Aug 17, 2026
Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry
Published on: June 26, 2018
[Dynamic changes of cytokines in G-CSF mobilized peripheral blood]
Cai-xia Li1, De-pei Wu, Wei-rong Chang
1Suzhou Medical Biotechnology Institute, Suzhou University, Suzhou 215007, China.
Insights
Interleukin-8 (IL-8) and adhesion molecules sICAM-1 and sVCAM-1 increase during stem cell mobilization. Their levels correlate with CD34+ cell counts, suggesting IL-8 and sVCAM-1 may predict stem cell yield.
Area of Science:
- Hematology
- Immunology
- Biochemistry
Context:
- Peripheral blood stem cell (PBSC) harvesting relies on effective mobilization.
- Mobilization involves dynamic biological processes influencing cell yields.
- Biomarkers are needed to predict and optimize PBSC harvesting outcomes.
Purpose:
- To investigate the levels and significance of IL-8, sICAM-1, and sVCAM-1 in peripheral blood during PBSC mobilization.
- To correlate these markers with hematopoietic cell counts and colony-forming units.
Summary:
- Plasma levels of IL-8, sICAM-1, and sVCAM-1 significantly increased during PBSC mobilization.
- IL-8 and sVCAM-1 concentrations paralleled the numbers of CD34+ cells, white blood cells, and platelets.
- Colony formation assays also showed correlation with IL-8 and sVCAM-1 levels.
Impact:
- Findings suggest IL-8 and sVCAM-1 dynamic changes can serve as predictive markers for CD34+ cell yield.
- This could lead to optimized mobilization strategies and improved PBSC harvesting efficiency.
- Understanding these inflammatory markers' roles may enhance patient stratification for stem cell transplantation.
Objective:
To investigate the level and significance of interleukin-8 (IL-8), soluble intercellular adhesion molecule (sICAM-1) and soluble vascular cell adhesion molecule-1 (sVCAM-1) in patients peripheral blood (PB) during mobilization for peripheral blood stem cells harvesting.
Methods:
The levels of IL-8, sICAM-1 and sVCAM-1 in patients were dynamically assayed by ELISA during the mobilization procedure and the number of CD(34)(+) cell, white blood cell (WBC) and platelet (BPC) by flow cytometric analysis and hematometry respectively. Colony formation was assayed by using semisolid methycellulose culture.
Results:
There was a significant increase in plasma levels of IL-8 and both adhesion molecules [IL-8 (247.4 +/- 84.2) microg/L (P < 0.01); sICAM-1 (530.3 +/- 286.1) microg/L (P = 0.002 7); sVCAM-1 (575.3 +/- 350.4) microg/L (P = 0.001 3)] during the mobilization process; furthermore, IL-8 and sVCAM-1 concentration in the patient's plasma was paralleled to the numbers of CD(34)(+) cell, CFU-GM, WBC and BPC (P < 0.001).
Conclusion:
The levels of IL-8, sICAM-1 and sVCAM-1 in the patient's plasma were correlated to the PB number of CD(34)(+) cells, CFU-GM, WBC and BPC during the mobilization process. It suggested that analysis of IL-8, and sVCAM-1 dynamic changes may serve as markers for CD(34)(+) cells.
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