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Updated: Aug 30, 2026
Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood
Published on: February 6, 2018
Production and the characterization of monoclonal antibody against CD43, K06
1Department of Pathology, Kangwon National University College of Medicine, Chuncheon, Korea.
Insights
A new monoclonal antibody, K06, recognizes a novel epitope on the CD43 molecule. This K06 epitope
Area of Science:
- Immunology
- Molecular Biology
- Cell Biology
Background:
- CD43 is a transmembrane glycoprotein expressed on various hematopoietic cells.
- Understanding CD43 epitopes is crucial for cell differentiation and immune responses.
Purpose of the Study:
- To characterize a novel monoclonal antibody, K06, targeting human CD43.
- To investigate the expression and function of the K06-defined CD43 epitope.
Main Methods:
- Development and characterization of anti-CD43 monoclonal antibody K06.
- Immunohistochemical analysis of lymphoid organs and peripheral blood.
- Biochemical analysis of CD43 antigen.
- Apoptosis induction studies in leukemic cell lines.
Main Results:
- K06 antibody recognizes a novel CD43 epitope with distinct reactivity patterns in lymphoid organs.
- The K06 epitope is highly expressed on cortical thymocytes, platelets, and myeloid cells.
- Biochemical studies show K06 recognizes a glycosylated moiety of CD43.
- CD43 ligation via the K06 epitope induces apoptosis in Molt-4 cells.
Conclusions:
- The K06 antibody identifies a novel CD43 epitope with potential roles in T-cell development.
- This epitope may be involved in regulating apoptosis in certain cell types.
Abstract:
A monoclonal antibody against human CD43 has been developed and designated as K06. Its reactivity in the lymphoid organs was different from that of known anti-CD43 monoclonal antibodies suggesting that this may recognize a novel epitope of human CD43 molecule. The CD43 epitope detected by anti-K06 monoclonal antibody was highly expressed in cortical thymocytes, platelets, and myeloid cells of normal peripheral blood, and its reactivity was comparable to that of known anti-CD43 monoclonal antibodies. However, the density of this epitope was lower in the medullary thymocytes. Biochemical studies indicated that anti-K06 monoclonal antibody could recognize glycosylated moiety of CD43 antigen. The expression profile of anti-K06 monoclonal antibody in several cell lines was somewhat different from that of known anti-CD43 antibodies. In addition, CD43 ligation through the K06 epitope appeared to induce apoptosis in human leukemic cell line, Molt-4. We therefore assume that K06 epitope of human CD43 might have some role in T-cell development.
