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Positive correlation between in vivo oxidized LDL and LDL immune complexes
Hu Bing1, Junjun Wang, Chunni Zhang
1Department of Traditional Chinese Medicine, Jinling Hospital, Clinical School of Medical College, Nanjing University, Nanjing 210002, China.
Insights
Oxidized low-density lipoprotein (ox-LDL) and LDL immune complexes (IC) are elevated in coronary heart disease patients. These findings show a positive correlation between in vivo ox-LDL and circulating LDL-IC levels.
Area of Science:
- Cardiovascular Research
- Immunology
- Lipid Metabolism
Background:
- Oxidized low-density lipoprotein (ox-LDL) plays a role in atherosclerosis.
- LDL immune complexes (IC) are implicated in cardiovascular disease pathogenesis.
Purpose of the Study:
- To investigate the relationship between circulating oxidized low-density lipoprotein (ox-LDL) and LDL immune complexes (IC).
Main Methods:
- Sandwich ELISA was used to detect both LDL-IC and ox-LDL.
- Levels were quantified in 60 coronary heart disease (CHD) patients and 50 control subjects.
Main Results:
- Patients with CHD exhibited significantly higher plasma ox-LDL and LDL-IC levels compared to controls.
- LDL-IC and ox-LDL levels were positively correlated with various lipid parameters, including total cholesterol, LDL cholesterol, and apolipoprotein B.
- A significant positive correlation was observed between ox-LDL and LDL-IC levels (r = 0.313, P < 0.005).
Conclusions:
- In vivo oxidized LDL is positively associated with circulating levels of LDL immune complexes.
- These findings suggest a potential link between LDL oxidation and immune complex formation in the context of cardiovascular disease.
Objectives:
To investigate the possible relationship between oxidized low-density lipoprotein (ox-LDL) and LDL immune complexes (IC).
Methods:
Both LDL-IC and ox-LDL were detected by sandwich ELISA. The levels were also studied in 60 patients with coronary heart disease (CHD) and 50 control subjects.
Results:
Compared to controls, both the plasma ox-LDL concentrations (595.5 +/- 194.8 vs. 440.3 +/- 175.0 microg/l, P < 0.001) and LDL-IC levels (2.74 +/- 0.73 vs. 1.38 +/- 0.78 AU, P < 0.001) in the patients with CHD were significantly increased. The relationships between LDL-IC, ox-LDL levels, and other lipid parameters in all the studied subjects (n = 100) were analyzed. LDL-IC levels were positively correlated with TC, TG, LDL-C, lipoprotein(a) [Lp(a)], and apolipoprotein B (apoB) concentration, while negatively correlated with apoA1 concentration, respectively. Similarly, ox-LDL levels were also found positively correlated with TC, LDL-C, and apoB concentrations, respectively. Furthermore, a significantly positive correlation between ox-LDL and LDL-IC levels was found (r = 0.313, P < 0.005).
Conclusion:
In vivo oxidized LDL positively correlates with circulating levels of LDL immune complexes.
