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Updated: Aug 8, 2026
Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging
Published on: April 8, 2016
A novel technology allowing immunohistochemical staining of a tissue section with 50 different antibodies in a single
Tomoko Furuya1, Kenzo Ikemoto, Shigeto Kawauchi
1Department of Pathology, Yamaguchi University School of Medicine, Ube, Japan.
Insights
A novel multiplex-immunostain chip (MI chip) simplifies tumor diagnosis by enabling 50-antibody immunohistochemical staining in one experiment. This cost-effective device reduces time and effort for pathologists.
Area of Science:
- Oncology
- Pathology
- Biotechnology
Background:
- Immunohistochemical (IHC) examination is crucial for tumor histological differential diagnosis.
- Current IHC methods can be time-consuming, labor-intensive, and expensive.
- There is a need for streamlined IHC techniques to improve diagnostic efficiency.
Purpose of the Study:
- To develop a novel device, the multiplex-immunostain chip (MI chip), to simplify IHC examination.
- To enable simultaneous staining of a tissue section with multiple antibodies.
- To reduce the time, effort, and cost associated with IHC analysis.
Main Methods:
- Development of a multiplex-immunostain chip (MI chip) consisting of a silicon rubber plate with 50 wells for antibodies.
- A tissue section slide is placed on the chip and secured with a clamp.
- The inverted chip-slide assembly allows antibodies to stain the tissue section in a single experiment.
Main Results:
- The MI chip allows IHC staining with 50 different antibodies in a single experiment.
- This technology significantly reduces the time, effort, and expense of IHC analysis.
- Pathologists can easily compare multiple antigen expressions on a single slide by changing microscopic fields.
Conclusions:
- The MI chip offers a unique and simplified approach to IHC staining.
- This cost-effective method requires no expensive instruments and has broad applications in tumor differential diagnosis and cell biology.
- The MI chip enhances diagnostic efficiency and facilitates comparative analysis of antigen expression.
Abstract:
Immunohistochemical (IHC) examination is frequently necessary for a histological differential diagnosis of tumors. To simplify IHC examination, we have developed a novel device called a "multiplex-immunostain chip (MI chip)." The chip is a panel of antibodies contained in a silicon rubber plate that consists of 50 2-mm-diameter wells. A tissue section slide is placed on the plate and is fastened tightly with a specially designed clamp. The plate with the slide is then turned upside down, which applies the antibodies to the section. This technology allows IHC staining of a tissue section with 50 different antibodies in a single experiment, reducing the time, effort, and expense of IHC analysis. In addition, it enables pathologists to compare expression of multiple antigens on a tissue section simply by changing microscopic fields on a single slide. These features are unique to the MI chip technology. The method requires no expensive instruments. This device can be used in various applications in differential diagnosis of tumors and the field of cell biology.
