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Cloning and characterization of an immunoglobulin A Fc receptor from cattle

H Craig Morton1, Richard J Pleass, Anne K Storset

  • 1Laboratory of Immunohistochemistry and Immunopathology (LIIPAT), Institute of Pathology, University of Oslo, Rikshospitalet, Oslo, Norway. craig.morton@labmed.uio.no

Immunology
|March 19, 2004
PubMed

Insights

Researchers cloned and characterized a novel bovine immunoglobulin A (IgA) Fc receptor (bFcalphaR). This discovery enhances understanding of IgA-Fc receptor interactions in cattle and related species.

Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • Immunoglobulin A (IgA) plays a crucial role in mucosal immunity.
  • Fc receptors mediate the interaction between antibodies and immune cells.
  • Characterization of bovine IgA Fc receptors is essential for understanding cattle immune responses.

Purpose of the Study:

  • To clone, sequence, and characterize a novel bovine IgA Fc receptor (bFcalphaR).
  • To investigate the functional binding properties of the identified bFcalphaR.
  • To determine the genetic relationship and chromosomal location of the bFcalphaR gene.

Main Methods:

  • Screening of a translated EST database using human CD89 sequence.
  • Polymerase chain reaction (PCR) amplification of full-length cDNA.
  • COS-1 cell transfection and binding assays with IgA and IgG coated beads.

Main Results:

  • Identification and cloning of the full-length bFcalphaR cDNA (873 nucleotides).
  • Transfected cells bound to bovine and human IgA, but not IgG.
  • Predicted 269 amino-acid transmembrane glycoprotein with two extracellular domains.
  • bFcalphaR gene is evolutionarily related to CD89 and maps to bovine chromosome 18.

Conclusions:

  • The bFcalphaR is a functional IgA Fc receptor expressed in cattle.
  • Understanding bFcalphaR provides insights into IgA-mediated immunity in bovines.
  • This research facilitates further study of Fc receptors across species.

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