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Determination of laboratory reference ranges for lymphocyte immunophenotype subsets in healthy adult Japanese using
H Kyle Webster1, Yohko Kawai, Douslas Haney
1BD Biosciences Immunocytometry Systems (BD Biosciences), San Jose, California, USA.
Insights
This study establishes reference ranges for lymphocyte subsets in Japanese adults. Findings highlight ethnic variations in lymphocyte values, suggesting population-specific ranges are crucial for accurate interpretation.
Area of Science:
- Immunology
- Hematology
- Clinical Chemistry
Background:
- Accurate lymphocyte subset reference ranges are essential for clinical diagnostics and research.
- Existing reference values may not be universally applicable across diverse ethnic populations.
- Flow cytometry is a key technique for enumerating lymphocyte populations.
Purpose of the Study:
- To establish normative values for lymphocyte immunophenotype subsets in a healthy Japanese adult population.
- To determine reference ranges for T, B, and NK cell percentages and absolute counts.
- To investigate age and gender-specific differences in lymphocyte subsets and activation markers.
Main Methods:
- Utilized a two-color immunophenotyping panel for lymphocyte subset analysis.
- Employed consistent quality control methodologies throughout the study.
- Applied flow cytometry techniques to quantify lymphocyte populations and activation markers.
Main Results:
- Established reference ranges for key lymphocyte subsets (T, B, NK cells) in Japanese adults.
- Identified significant differences in lymphocyte subset values based on age and gender.
- Demonstrated ethnic variations in lymphocyte reference ranges when compared to a Thai population study.
Conclusions:
- Normative lymphocyte reference values are population-specific and cannot be directly extrapolated between ethnic groups.
- Age and gender significantly influence lymphocyte subset distributions.
- This study provides crucial data for the accurate interpretation of flow cytometry results in Japanese individuals.
Abstract:
This study represents a comprehensive evaluation of normative values for lymphocyte immunophenotype subsets using flow cytometry techniques in a Japanese population. Lymphocyte reference ranges were determined for percentage and absolute count of T, B, and NK cells in healthy adult Japanese using an extensive two-color immunophenotyping panel and consistently applied quality control methodology. Reference values were also determined for activation markers on CD3+ lymphocytes CD3+/CD25+, CD3+/CD38+ and CD3+/HLA-DR+. Differences in age and gender were observed for specific lymphocyte subsets. Comparison of the Japanese study with a Thai multi-center study that used similar methodology also demonstrated ethnic differences in lymphocyte reference ranges. The results in this study strongly suggest that reference values derived from studies in one population may not be applied to another population even when similar protocols for reagents, instruments and procedures are used although such studies do appear useful for epidemiological comparisons.
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