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Immune systems biology: immunoprofiling of cells and molecules

Aaron B Kantor1, Susan E Alters, Karen Cheal

  • 1SurroMed, Inc., 1430 O'Brien Drive, Menlo Park, CA 94025, USA. akantor@surromed.com

Biotechniques
|March 25, 2004
PubMed

Insights

A new laser scanning cytometer, SurroScan, enables high-throughput immunoprofiling of small fluid samples. This technology aids in understanding immune responses and disease monitoring by analyzing cellular and molecular variables.

Area of Science:

  • Immunology
  • Biotechnology
  • Medical Diagnostics

Background:

  • White blood cells and their products are crucial indicators of health and disease.
  • Current methods may require large sample volumes or extensive sample preparation.
  • There is a need for high-throughput, automated analysis of immune markers.

Purpose of the Study:

  • To introduce and validate the SurroScan microvolume laser scanning cytometer.
  • To demonstrate the system's capability for comprehensive immunoprofiling.
  • To assess immune response characteristics in patient samples.

Main Methods:

  • Development of the SurroScan microvolume laser scanning cytometer.
  • Utilizing antibody-based assays for immunoprofiling.
  • Analyzing small sample volumes (approx. 1 mL) without cell purification.
  • High-throughput, automated data capture and analysis.

Main Results:

  • The SurroScan system successfully immunoprofiled hundreds of variables from small fluid samples.
  • Demonstrated utility in surveying patient samples before and after glucocorticosteroid administration.
  • Identified both expected and novel immune response characteristics.

Conclusions:

  • SurroScan offers a high-throughput, robust platform for microvolume immunoprofiling.
  • This technology facilitates detailed analysis of immune responses.
  • It complements genomic and proteomic approaches for disease prediction and monitoring.

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