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Published on: January 25, 2017
Snapper (Pagrus auratus) leucocyte proliferation is synergistically enhanced by simultaneous stimulation with LPS and
Richard N Morrison1, A Bruce Lyons, Barbara F Nowak
1School of Aquaculture and CRC for Aquaculture, Tasmanian Aquaculture and Fisheries Institute, University of Tasmania, Locked Bag 1-370, Launceston, Tasmania 7250, Australia. rmorriso@utas.edu.au
Insights
Snapper leucocytes communicate upon stimulation, similar to mammals. Different cell types influence each other
Area of Science:
- Immunology
- Marine Biology
- Fish Health
Background:
- Leucocyte communication is vital in mammalian immunity.
- Understanding fish leucocyte interactions is crucial for aquaculture and conservation.
Purpose of the Study:
- To investigate potential communication between snapper leucocytes.
- To determine if mitogen stimulation induces leucocyte proliferation and interaction in snapper.
Main Methods:
- Peripheral blood leucocytes (PBLs) from snapper were isolated.
- Cell surface immunoglobulin (IgM) expression, intracellular fluorescence, and flow cytometry were used.
- Cells were stimulated with phytohaemagglutinin (PHA) or lipopolysaccharide (LPS) independently and simultaneously.
Main Results:
- Both mIg(-) and mIg(+) snapper leucocytes proliferated upon PHA or LPS stimulation.
- Simultaneous PHA and LPS stimulation resulted in significantly higher proliferation than individual stimuli.
- This suggests synergistic interactions between different leucocyte subsets.
Conclusions:
- Snapper leucocytes exhibit communication pathways similar to mammals.
- Leucocyte subsets can influence each other's response to mitogenic stimulation.
- These findings have implications for understanding fish immune responses and health.
Abstract:
Important channels of communication between mammalian leucocytes have long been recognised. Here, data are reported that suggest similar integrations may occur between snapper leucocytes upon mitogen stimulation. Cell surface immunoglobulin (IgM) expression was used in conjunction with intracellular fluorescence staining and flow cytometry to differentiate proliferating peripheral blood leucocyte subsets (PBLs). Independent activation using phytohaemagglutinin (PHA) or lipopolysaccharide (LPS) drove both mIg(-)and mIg(+)cells into cycle. It is not known if the proliferation of mIg(+)cells was mediated by a mutually exclusive effect of the mitogen on each cell population, cognate cellular interaction or a soluble growth factor. Simultaneous activation of PBLs with PHA and LPS consistently induced significantly more cells to proliferate than the sum of proliferating cells stimulated solely with PHA or LPS. Together, the results suggest that different leucocyte subsets have the capability to influence their respective responses to mitogenic stimulation. Therefore, like in the mammalian immune system, communication may occur between snapper leucocyte subsets.
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