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Leukaemia/lymphoma cell microparticles in childhood mature B cell neoplasms
S Savaşan1, M Büyükavci, S Buck
1Children's Hospital of Michigan, Division of Hematology/Oncology, Barbara Ann Karmanos Cancer Institute, Wayne State University, Detroit, MI 48201, USA. ssavasan@med.wayne.edu
Insights
Leukaemia/lymphoma cell microparticles are commonly released by mature B cell neoplasms. This study investigated their presence and found them distinct from apoptotic bodies, warranting further research into their clinical significance.
Area of Science:
- Hematology
- Oncology
- Cell Biology
Background:
- Leukaemia/lymphoma cell microparticles were observed in a patient with Burkitt's leukaemia.
- The presence and nature of these microparticles in various leukaemia/lymphoma subtypes were not well-characterized.
Purpose of the Study:
- To investigate the occurrence of leukaemia/lymphoma cell microparticles in leukaemia/lymphoma samples.
- To differentiate these microparticles from apoptotic bodies.
Main Methods:
- Flow cytometric immune phenotyping and apoptosis analysis were performed on bone marrow samples.
- Microparticles were isolated and analyzed.
- Retrospective analysis of 225 leukaemia/lymphoma cases with prior immune phenotyping data.
Main Results:
- Leukaemia/lymphoma cell microparticles were detected by flow cytometry and distinguished from apoptotic bodies.
- Microparticle release was observed in all studied mature B cell neoplasms.
- The phenomenon was rare in precursor B cell disorders and acute myeloid leukaemia.
Conclusions:
- The generation of leukaemia/lymphoma cell microparticles is a common event in mature B cell neoplasms.
- Further investigation into the pathogenesis and clinical implications of these microparticles is necessary.
Aims:
Because of the observation of an abundance of leukaemia/lymphoma cell microparticles in the bone marrow aspiration sample of a patient with Burkitt's leukaemia at diagnosis, the occurrence of this phenomenon in leukaemia/lymphoma samples with available immune phenotyping data was investigated retrospectively.
Methods:
Flow cytometric immune phenotyping and spontaneous apoptosis analysis of the bone marrow mononuclear cell preparation of the index case were performed. Microparticles isolated form the bone marrow sample were also studied for the presence of leukaemia/lymphoma cell microparticles. List mode analysis of 225 cases of acute leukaemia or lymphoma with previously performed immune phenotyping was also carried out.
Results:
The presence of leukaemia/lymphoma cell microparticles could be detected by flow cytometry and they were found to be different from apoptotic bodies. Leukaemia/lymphoma cell microparticles were released in all cases of mature B cell neoplasms studied, although this phenomenon was rare in precursor B cell disorders and acute myeloid leukaemia.
Conclusions:
The generation of leukaemia/lymphoma cell microparticles in mature B cell neoplasms appears to be a common phenomenon. The pathogenesis and clinical implications must be investigated.
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