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A polymeric immunoglobulin receptor-like milk protein with inhibitory activity on human immunodeficiency virus type 1

T B Ng1, X Y Ye

  • 1Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.

The International Journal of Biochemistry & Cell Biology
|August 18, 2004
PubMed

Insights

A novel bovine milk protein, similar to polymeric immunoglobulin receptor, was isolated. This protein inhibits HIV-1 reverse transcriptase (RT) but lacks ribonuclease activity.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Immunology

Background:

  • Bovine milk contains various proteins with potential biological activities.
  • The polymeric immunoglobulin receptor (pIgR) plays a role in mucosal immunity.
  • Investigating milk proteins can reveal novel therapeutic or research tools.

Purpose of the Study:

  • To isolate and characterize an 85 kDa protein from bovine milk with sequence homology to pIgR.
  • To evaluate the protein's inhibitory activity against HIV-1 reverse transcriptase (RT).
  • To assess the protein's ribonuclease activity and its effect on cell-free translation.

Main Methods:

  • Protein isolation from bovine acid whey using ammonium sulfate precipitation.
  • Chromatographic purification including CM-Sepharose and Mono S.
  • Enzyme inhibition assays for HIV-1 RT, ribonuclease activity, and cell-free translation.

Main Results:

  • An 85 kDa protein with an N-terminal sequence resembling pIgR was successfully isolated.
  • The isolated protein demonstrated significant inhibition of HIV-1 RT with an IC(50) of 4.8 microM.
  • No ribonuclease activity or inhibition of cell-free translation was observed.

Conclusions:

  • A novel bovine milk protein with pIgR-like characteristics was identified.
  • This protein exhibits specific inhibitory activity against HIV-1 RT.
  • The findings suggest potential applications for this milk protein in antiviral research or therapy.

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