Related Experiment Video
Updated: Aug 22, 2026
ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Method for Mycotoxin Detection
Published on: October 23, 2009
[A novel microbeads-based chemiluminescence immunoassay for detecting human thyrotropin]
Yong-ning Wang1, Rong-xiu Li, Cheng-wu Li
1Technical Institute of Physics and Chemistry, Chinese Academy of Science, Beijing 100101, China.
Insights
A new, low-cost microbeads-based chemiluminescence immunoassay (CLEIA) accurately detects human thyrotropin (TSH). This sensitive assay shows promise for widespread clinical use in TSH measurement.
Area of Science:
- Biochemistry
- Immunology
- Analytical Chemistry
Context:
- Thyrotropin (TSH) is a crucial biomarker for thyroid function.
- Accurate TSH measurement is essential for diagnosing thyroid disorders.
- Existing assays may be costly or lack sensitivity.
Purpose:
- To develop a cost-effective and highly sensitive microbeads-based chemiluminescence immunoassay (CLEIA) for human TSH detection.
Summary:
- The assay utilizes FITC-labeled and alkaline phosphatase-labeled monoclonal antibodies (mAbs) for TSH capture.
- Immuno-magnetic beads conjugated with anti-FITC antibody isolate the captured TSH.
- Chemiluminescence detection with adamantane amine quantifies TSH, achieving a sensitivity of 0.004 mIU/L.
Impact:
- The developed CLEIA demonstrates excellent sensitivity, precision (intra-assay CV 7.45%, inter-assay CV 10.45%), and recovery (91.4%-102.4%).
- Results correlate well with the ACS-180 system, indicating clinical validity.
- This low-cost, sensitive, specific, and stable assay has significant potential for clinical applications in TSH testing.
Aim:
To establish a low cost and sensitive microbeads-based chemiluminescence immunoassay (CLEIA) for detecting human thyrotropin.
Methods:
Thyrotropin in sera was captured by an alkaline phosphase-labeled mAb against TSH beta subunit and a FITC-labeled mAb against TSH alpha subunit. Captured thyrotropin was then isolated with immuno-magnetic beads conjugated with anti-FITC antibody and then quantified by chemiluminescence using adamanatane amine as luminescent substrate.
Results:
The sensitivity of this assay is 0.004 mIU/L. The intra-assay CV and the inter-assay CV of the assay were 7.45% and 10.45%, respectively. The recovery rate was 91.4% -102.4%. The examination result of the assay correlates well with that of ACS-180 system.
Conclusion:
The CLELA for detecting TSH is low-cost, sensitive, specific and stable, and therefore may have a promising prospect in clinical application.
