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Cell cycle distribution of CD4+ lymphocytes in HIV-1-infected subjects

Mariana Melillo Sauer Chaves1, Esper Georges Kallas

  • 1Infectious Diseases Division, Federal University of São Paulo, São Paulo, Brazil.

Cytometry. Part B, Clinical Cytometry
|October 12, 2004
PubMed

Insights

HIV-1 infection alters CD4(+) lymphocyte cell cycle, increasing S phase and decreasing G(1) phase. This finding may help understand CD4(+) T-cell depletion in HIV patients.

Area of Science:

  • Immunology
  • Virology
  • Cell Biology

Background:

  • Apoptosis is a potential cause of CD4(+) T-lymphocyte loss in human immunodeficiency virus (HIV) infection.
  • HIV infection may disrupt normal lymphocyte cell cycle distribution.
  • This study investigates CD4(+) and CD8(+) lymphocyte cell cycle in HIV-infected individuals.

Purpose of the Study:

  • To evaluate the cell cycle distribution of CD4(+) and CD8(+) lymphocytes in HIV-1-infected subjects.
  • To compare cell cycle analysis methods for lymphocyte evaluation.

Main Methods:

  • Two methods for lymphocyte identification were assessed: magnetic beads and concurrent staining.
  • Propidium iodide DNA labeling was used for cell cycle analysis.
  • The selected method was applied to HIV-1 patients and controls.

Main Results:

  • No significant difference was found between magnetic bead and concurrent staining methods, though magnetic beads showed higher variability.
  • HIV-1 patients exhibited a higher proportion of CD4(+) lymphocytes in S phase (2.69% vs. 1.19%) and a decrease in G(1) phase (96.11% vs. 98.10%).
  • These cell cycle changes were not observed in CD8(+) lymphocytes and did not correlate with T-lymphocyte counts or viral load.

Conclusions:

  • A novel approach for evaluating lymphocyte cell cycle distribution was developed and applied to HIV-1 infection.
  • The findings may enhance understanding of CD4(+) T-lymphocyte depletion in HIV-1 patients.
Abstract

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