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The monocyte interleukin-2 receptor light chain: production of cell-associated and soluble interleukin-2 receptor by

E M Kniep1, I Strelow, M L Lohmann-Matthes

  • 1Abteilung für Immunobiologie, Fraunhofer Institut für Toxikologie, Hannover, Germany.

Immunology
|February 1, 1992
PubMed

Insights

Monocytes stimulated with lipopolysaccharide (LPS) produce significant amounts of soluble interleukin-2 receptor light chain (p55 IL-2R), primarily in the cytoplasm. This soluble form is likely destined for secretion, not membrane attachment.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Interleukin-2 receptor (IL-2R) is crucial for T lymphocyte activation.
  • Monocytes, a type of white blood cell, play a role in immune responses.

Purpose of the Study:

  • To investigate the production and localization of the p55 IL-2R subunit in monocytes upon stimulation.
  • To compare IL-2R production in monocytes versus T lymphocytes.

Main Methods:

  • Monocytes were stimulated with lipopolysaccharide (LPS) and recombinant interferon-gamma.
  • Soluble IL-2R in supernatants was quantified.
  • Cell-associated p55 IL-2R was analyzed using cell fractionation and subcellular localization.
  • Gene expression of p55 IL-2R was assessed.

Main Results:

  • LPS stimulation induced monocytes to produce significant amounts of soluble p55 IL-2R, exceeding that of activated T lymphocytes.
  • Cell-associated p55 IL-2R increased in the first 24 hours and then stabilized.
  • The majority of cell-associated p55 IL-2R was found in the cytoplasm, not membrane-bound.
  • Interferon-gamma did not enhance p55 IL-2R production.

Conclusions:

  • Monocyte-associated p55 IL-2R exists significantly in a soluble cytoplasmic form, suggesting it is newly synthesized for secretion.
  • Monocytes are a notable source of soluble IL-2R, particularly after LPS stimulation.

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