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Published on: December 26, 2017
[In vitro effect of interferon alpha on proliferation of human marrow fibroblast]
1Department of Hematology, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou 310016, China.
Insights
Interferon alpha (IFN-alpha) combined with transforming growth factor-beta1 (TGF-beta1) synergistically inhibits platelet-derived growth factor-AB (PDGF-AB) induced human marrow fibroblast proliferation. This effect was observed specifically at the S phase of the cell cycle in vitro.
Area of Science:
- Cell Biology
- Hematology
- Immunology
Background:
- Human marrow fibroblasts play a role in the bone marrow microenvironment.
- Growth factors like PDGF-AB and TGF-beta1 influence fibroblast proliferation.
- Interferon alpha (IFN-alpha) is a cytokine with known immunomodulatory effects.
Purpose of the Study:
- To investigate the impact of interferon alpha (IFN-alpha) on the proliferation of human marrow fibroblasts.
- To determine the combined effects of IFN-alpha with other growth factors on fibroblast proliferation.
Main Methods:
- Human marrow fibroblasts were isolated from patients with iron deficiency anemia.
- Cells were treated with platelet-derived growth factor-AB (PDGF-AB), transforming growth factor-beta1 (TGF-beta1), or IFN-alpha2b, individually or in combination.
- Cell proliferation was assessed using MTT assays, and cell cycle analysis was performed via flow cytometry.
Main Results:
- PDGF-AB and TGF-beta1 stimulated fibroblast proliferation, with PDGF-AB being more potent.
- IFN-alpha2b partially suppressed PDGF-AB-induced proliferation by 7.62%.
- The combination of IFN-alpha2b and TGF-beta1 demonstrated synergistic suppression of both basal and PDGF-AB-induced fibroblast proliferation, particularly affecting the S phase.
Conclusions:
- IFN-alpha2b and TGF-beta1 exhibit synergistic inhibitory effects on PDGF-AB-induced human marrow fibroblast proliferation.
- This synergistic suppression occurs at the S phase of the cell cycle in vitro.
- These findings suggest a potential role for combined IFN-alpha and TGF-beta1 in modulating fibroblast activity within the bone marrow.
Objective:
To explore the effect of interferon alpha (IFN-alpha) on proliferation of human marrow fibroblasts.
Methods:
Marrow fibroblasts were isolated from patients with iron deficiency anemia. Platelet derived growth factor-AB (PDGF-AB), transforming growth factor-beta1 (TGF-beta1) or INF-alpha2b was added in the cell cultures each alone or in combinations. Cell proliferation and cell cycle of fibroblasts were detected by MTT and flow cytometry, respectively.
Results:
PDGF-ABqa (1 approximately 50 ng/ml) or TGF-beta1 (0.01 approximately 1 ng/ml) could stimulate the proliferation of the fibroblasts, while PDGF-AB was more effective (P < 0.01). IFN-alpha2b partially suppressed PDGF-AB induced proliferation of fibroblasts,the inhibition rate was 7.62%. IFN-alpha2b and TGF-beta1 combination suppressed synergistically not only the proliferation of the fibroblasts (P < 0.01) but also the PDGF-AB induced-proliferation (P < 0.01) at S phase.
Conclusion:
IFN-alpha2b combined with TGF-beta1 could synergistically suppress the PDGF-AB induced proliferation of fibroblasts at S phase in vitro.
