Related Experiment Video

Updated: Aug 9, 2026

Quantifying Human Monocyte Chemotaxis In Vitro and Murine Lymphocyte Trafficking In Vivo
08:38

Quantifying Human Monocyte Chemotaxis In Vitro and Murine Lymphocyte Trafficking In Vivo

Published on: October 30, 2017

The human cytokine I-309 is a monocyte chemoattractant

M D Miller1, M S Krangel

  • 1Department of Microbiology and Immunology, Duke University Medical Center, Durham, NC 27710.

Proceedings of the National Academy of Sciences of the United States of America
|April 1, 1992
PubMed

Insights

The human cytokine I-309, secreted by T lymphocytes, specifically attracts and activates monocytes. This inflammatory mediator increases intracellular calcium in monocytes, highlighting its role in immune responses.

Area of Science:

  • Immunology
  • Molecular Biology
  • Biochemistry

Background:

  • The human cytokine I-309 is a glycoprotein secreted by activated T lymphocytes.
  • It is structurally related to several inflammatory cytokines, suggesting a role in immune responses.

Purpose of the Study:

  • To investigate the biological activities of the I-309 protein.
  • To characterize the function of I-309 as an inflammatory mediator.

Main Methods:

  • Production of a stable Chinese hamster ovary cell transfectant (CDI.10) secreting I-309.
  • Purification of recombinant I-309 glycoprotein using heparin-Sepharose chromatography and reverse-phase HPLC.
  • In vitro chemotaxis assays and measurement of cytoplasmic free calcium concentration in human monocytes, lymphocytes, and neutrophils.

Main Results:

  • Purified recombinant I-309 was biochemically indistinguishable from natural I-309.
  • Recombinant I-309 stimulated the migration of human monocytes but not neutrophils.
  • The protein increased cytoplasmic free calcium concentration in human peripheral blood monocytes, but not in lymphocytes or neutrophils.

Conclusions:

  • The I-309 gene encodes an inflammatory mediator.
  • I-309 specifically stimulates human monocytes, suggesting a key role in monocyte-directed inflammatory processes.

More Related Videos

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes11:48

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Published on: May 31, 2018

Quantification of Monocyte Chemotactic Activity In Vivo and Characterization of Blood Monocyte Derived Macrophages09:57

Quantification of Monocyte Chemotactic Activity In Vivo and Characterization of Blood Monocyte Derived Macrophages

Published on: August 12, 2019

Related Concept Videos

Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...