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Updated: Aug 11, 2026
Analyzing the Functions of Mast Cells In Vivo Using 'Mast Cell Knock-in' Mice
Published on: May 27, 2015
The CD200 receptor is a novel and potent regulator of murine and human mast cell function
Holly M Cherwinski1, Craig A Murphy, Barbara L Joyce
1DNAX Research Institute, Palo Alto, CA 94304, USA.
Insights
CD200R, an inhibitory receptor, is expressed on mast cells and potently inhibits their activation. This finding offers a novel target for regulating mast cell-driven diseases.
Area of Science:
- Immunology
- Cell Biology
Background:
- CD200R is an inhibitory receptor primarily found on myeloid cells.
- Previous studies suggested CD200R regulates inflammatory immune responses.
Purpose of the Study:
- To confirm CD200R expression on mast cells.
- To investigate CD200R's role in mast cell activation and degranulation.
Main Methods:
- Utilized mouse and human mast cells.
- Employed agonist antibodies and ligand to engage CD200R.
- Assessed mast cell degranulation and cytokine secretion.
- Investigated CD200R function in vitro and in vivo.
Main Results:
- CD200R is definitively expressed on both mouse and human mast cells.
- Engagement of CD200R significantly inhibits mast cell degranulation and cytokine release.
- Inhibition of FcepsilonRI activation by CD200R occurred independently of co-ligation.
- CD200R lacks a typical ITIM motif, distinguishing it from other myeloid inhibitory receptors.
Conclusions:
- CD200R is a novel and potent inhibitory receptor on mast cells.
- CD200R signaling effectively suppresses mast cell activation.
- CD200R presents a potential therapeutic target for mast cell-mediated inflammatory diseases.
Abstract:
CD200R is a member of the Ig supergene family that is primarily expressed on myeloid cells. Recent in vivo studies have suggested that CD200R is an inhibitory receptor capable of regulating the activation threshold of inflammatory immune responses. Here we provide definitive evidence that CD200R is expressed on mouse and human mast cells and that engagement of CD200R by agonist Abs or ligand results in a potent inhibition of mast cell degranulation and cytokine secretion responses. CD200R-mediated inhibition of FcepsilonRI activation was observed both in vitro and in vivo and did not require the coligation of CD200R to FcepsilonRI. Unlike the majority of myeloid inhibitory receptors, CD200R does not contain a phosphatase recruiting inhibitory motif (ITIM); therefore, we conclude that CD200R represents a novel and potent inhibitory receptor that can be targeted in vivo to regulate mast cell-dependent pathologies.
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