Monoclonal antibody 83D4 immunoreactivity in human tissues: cellular distribution and microcytophotometric analysis of immunoprecipitates on tissue sections
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Insights
A new monoclonal antibody, MAb 83D4, shows promise for grading breast cancer. It detects cancer cells in various tissues and can identify microvascular invasions and micrometastases, aiding in early detection and prognosis.
Area of Science:
- Oncology
- Immunohistochemistry
- Biomarker Discovery
Background:
- Monoclonal antibodies are crucial tools in cancer diagnostics.
- Accurate grading of breast cancer is essential for effective treatment planning.
Purpose of the Study:
- To evaluate the utility of a novel monoclonal antibody, MAb 83D4, for breast cancer diagnosis and grading.
- To assess the correlation of MAb 83D4 reactivity with various tumor characteristics.
Main Methods:
- Immunocytochemical assays on cell cultures and human tissues using MAb 83D4.
- Comparison of frozen and paraffin-embedded breast tissue samples.
- Computer-assisted microcytophotometry (SAMBA) for evaluating immunoprecipitates.
- Correlation analysis with tumor cell DNA index, ploidy, Ki67, hormone receptors, and HER-2/neu.
Main Results:
- MAb 83D4 reacted specifically with breast cancer cell lines, not normal breast cells.
- Antigen concentration increased with malignancy but was independent of DNA content, hormone receptors, or HER-2/neu.
- Immunostaining was consistent between frozen and paraffin-embedded sections.
Conclusions:
- MAb 83D4 is a potential tool for routine immunohistochemical grading of breast cancer.
- It can facilitate the detection of microvascular invasion and blood-borne micrometastases.
- This antibody may improve early detection and prognostic assessment in breast cancer patients.
Abstract:
Immunocytochemical assays were performed on cell cultures as well as on a wide range of human tissues using a monoclonal antibody, MAb 83D4, produced by a murine hybridoma generated by immunization with a cell suspension from a paraffin block of human breast carcinoma tissue. Frozen breast tissue samples (n = 49) were compared to fixed and paraffin-embedded samples (n = 62). Paraffin sections (n = 194) from a variety of human tissues were compared to breast immunoreactivity. Immunoprecipitates, resulting from positive reactions between 83D4 and Avidin Biotin Peroxidase, were evaluated by computer-assisted microcytophotometry (SAMBA). In some frozen breast samples (n = 27), 83D4 antigen distribution was correlated with tumor cell DNA index, ploidy balance, growth fraction (Ki67), hormone receptor (ER, PR) antigenic sites, NORsAg and oncoprotein pHER-2/neu cell content. MAb 83D4 reacted with 3 breast cancer cells lines (MCF7, T47D and H466B) but not with normal epithelial breast cells in culture. The immunostaining in frozen paraffin sections from breast were similar. Like most normal tissues, normal breast did not react with 83D4. Cellular MAb 83D4 antigen concentration increases with the degree of malignancy but is independent of DNA nuclear content, ER, PR, growth fraction and pHER-2neu in cancers. These results suggested that routine immunohistochemical procedures using MAb 83D4 could facilitate the grading of breast cancer, in particular by allowing detection of microvascular invasions in the breast as well as at a distance and of blood-born micrometastases, especially in bone marrow.
