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Updated: Aug 8, 2026
Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
Published on: March 26, 2018
[Immunophenotypes in 115 patients with acute myeloid leukemia by multi-color flow cytometry]
Xing-Bing Wang1, Jun-Xia Yao, Jin-E Zheng
1Stem Cell Research and Application Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
Insights
Multiparameter flow cytometry aids in diagnosing acute myeloid leukemia (AML). Specific immunophenotypes correlate with French-American-British (FAB) classifications, improving AML diagnosis and subtyping.
Area of Science:
- Hematology
- Immunology
- Oncology
Context:
- Immunophenotyping is crucial for leukemia diagnosis and classification.
- Acute myeloid leukemia (AML) diagnosis benefits from detailed immunophenotypic analysis.
Purpose:
- To evaluate the role of multiparameter flow cytometry (MFC) in diagnosing AML.
- To correlate immunophenotypes with the French-American-British (FAB) classification of AML.
Summary:
- MFC and CD45/SSC gating analyzed surface and cytoplasmic antigens in 115 AML cases.
- CD38, CD13, CD7, CD19, CD2, CD14, CD56, HLA-DR, and MPO expression patterns were identified.
- Specific antigen expressions correlated with FAB subtypes (M(0), M(2), M(3), M(5)).
Impact:
- MFC is a reliable diagnostic technique for AML.
- Immunophenotypic correlations enhance AML classification accuracy.
- Findings support MFC's utility in differentiating AML subtypes based on FAB criteria.
Abstract:
Immunophenotyping has become common in the diagnosis and classification of leukemia. To evaluate the immunophenotype of acute myeloid leukemia (AML), multiparameter flow cytometry and CD45/SSC gating were used to analyze the surface and cytoplasmic antigen expressions in 115 cases of AML. The results were compared with the French-American-British (FAB) Cooperative Group classification to help define the best use and role of multiparameter flow cytometry in the diagnosis and proper classification of AML. The results showed that CD38, CD38 and CD13 were the most commonly expressed antigen (94.8%, 91.3% and 89.6%, respectively). CD7 was the most commonly expressed lymphoid antigen (20.2%), followed by CD19 (16.5%) and CD2 (15%). Some immunophenotypes correlated with FAB type, including increased frequency of CD2 in M(3); lack of HLA-DR, CD34 and CD56 expression in M(3); increased frequency of CD19 in M(2), CD14 and CD56 in M(5) and lack of MPO in M(0). In conclusion, multiparameter flow cytometry is a reliable technique in the diagnosis of AML, and some immunophenotypes correlate with FAB type.
