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Reference ranges of lymphocyte subsets of healthy adults in Turkey
Akgun Yaman1, Salih Cetiner, Filiz Kibar
1Department of Microbiology, Faculty of Medicine, Cukurova University, Balcali-Adana, Turkey. kyaman@cu.edu.tr
Insights
This study establishes reference ranges for lymphocyte subsets in healthy Turkish adults. These values are crucial for interpreting immune cell counts in clinical settings.
Area of Science:
- Immunology
- Hematology
- Clinical Pathology
Background:
- Accurate reference ranges for lymphocyte subsets are essential for diagnosing and monitoring various immunological conditions.
- Establishing population-specific reference values ensures accurate clinical interpretation.
Purpose of the Study:
- To determine the reference ranges for lymphocyte subsets in serologically HIV-negative healthy adults residing in Turkey.
- To provide a baseline for immunological assessments in the Turkish population.
Main Methods:
- Flow cytometry was utilized to analyze lymphocyte subsets in blood samples.
- The study included 220 healthy adults (105 female, 115 male) aged 18-80 years.
Main Results:
- Mean percentages and absolute counts for CD3, CD4, CD8, CD19, and CD56 lymphocyte subsets were determined.
- The CD4/CD8 ratio was calculated, with a mean of 1.68 +/- 0.43.
- No statistically significant gender-based differences were observed in lymphocyte subset values.
Conclusions:
- Immunophenotyping successfully established reference values for lymphocyte subsets in healthy Turkish adults.
- These established ranges serve as a vital resource for clinical immunology in Turkey.
Unlabelled:
The aim of this study was to determine the reference ranges of lymphocyte subsets in serologically HIV-negative healthy adults in Turkey.
Materials And Methods:
Blood samples from 220 healthy adults, 105 female and 115 male, collected into tubes containing EDTA were investigated for lymphocyte subsets using flow cytometry. The age range was 18-80 years (44.80 +/- 16.69).
Results:
The mean percentage and absolute values of the lymphocyte subsets were as follows: CD3: 72.70 +/- 8.44%, 1,680 +/- 528 cells/microl; CD4: 47.37 +/- 9.10%, 1,095 +/- 391 cells/microl; CD8: 28.99 +/- 5.99%, 669 +/- 239 cells/microl; CD19: 10.96 +/- 4.44%, 254 +/- 122 cells/microl and CD56: 7.03 +/- 3.26%, 161 +/- 92 cells/microl, respectively. The ratio of CD4/CD8 was 1.68 +/- 0.43. There was no statistically significant difference in the percentages and absolute values of lymphocyte subsets between the genders (p > 0.05).
Conclusion:
Immunophenotyping has been used to establish reference values of lymphocyte subsets in normal healthy adults in Turkey.
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