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A monocyte/macrophage antigen recognized by the four antibodies GHI/61, Ber-MAC3, Ki-M8 and SM4
K Pulford1, K Micklem, S McCarthy
1LRF Immunodiagnostics Unit, John Radcliffe Hospital, Headington, Oxford, U.K.
Insights
A novel monoclonal antibody, GHI/61, identifies monocytes and macrophages by targeting a unique intracellular antigen. This finding suggests a new CD group for GHI/61 and related antibodies.
Area of Science:
- Immunology
- Cell Biology
Background:
- Monocytes and macrophages are critical immune cells involved in various physiological and pathological processes.
- Characterization of cell surface and intracellular antigens aids in understanding immune cell subsets and functions.
Purpose of the Study:
- To describe a new monoclonal antibody, GHI/61, that specifically labels monocytes and tissue macrophages.
- To characterize the antigen recognized by GHI/61 and compare it with existing markers.
Main Methods:
- Biochemical analysis
- Flow cytometry
- Immunocytochemical studies
Main Results:
- Antibody GHI/61 recognizes an intracellular antigen of 130,000 MW (reduced) and 110,000 MW (unreduced).
- GHI/61 identifies the majority of monocytes and tissue macrophages.
- GHI/61 recognizes the same antigen as antibodies Ber-MAC3, Ki-M8, and SM4.
Conclusions:
- Antibodies GHI/61, SM4, Ber-MAC3, and Ki-M8 should be grouped into a new CD designation.
- The antigen recognized by GHI/61 is amenable to purification, enabling gene sequencing and isolation.
Abstract:
A new monoclonal antibody, GHI/61, which labels the majority of monocytes and tissue macrophages is described. This antibody recognizes an intracellular antigen of 130,000 MW (reduced) and 110,000 MW (unreduced). Using biochemical, flow cytometry and immunocytochemical studies antibody GHI/61 was shown to recognize the same antigen as the previously described antibodies Ber-MAC3, Ki-M8 and SM4. On the basis of the results obtained, antibodies GHI/61, SM4, Ber-MAC3 and Ki-M8 should form a new CD group at the next Leucocyte Typing Workshop. Since the antigen recognized by the antibody GHI/61 is relatively easy to purify, the sequencing and the isolation of the gene encoding this protein should be possible.
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