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Published on: March 18, 2017
Ginsenoside-Ro enhances cell proliferation and modulates Th1/Th2 cytokines production in murine splenocytes
Jun-li Yu1, De-qiang Dou, Xiao-hong Chen
1Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
Insights
Ginsenoside-Ro modulates immune responses in mouse cells. It enhances proliferation and IL-2 production while promoting Th2 cytokine IL-4 and suppressing Th1 cytokine IFN-gamma in activated splenocytes.
Area of Science:
- Immunology
- Pharmacology
- Natural Products
Background:
- Ginsenosides are active compounds from Panax ginseng.
- Understanding their immunomodulatory effects is crucial for therapeutic applications.
Purpose of the Study:
- To investigate the impact of ginsenoside-Ro on murine splenocyte proliferation.
- To analyze the effects of ginsenoside-Ro on cytokine production (IL-2, IFN-gamma, IL-4).
Main Methods:
- Cell proliferation assessed via [3H] thymidine incorporation.
- Cytokine levels (IL-2, IFN-gamma, IL-4) measured using ELISA.
- mRNA expression of IFN-gamma and IL-4 analyzed by RT-PCR.
Main Results:
- Ginsenoside-Ro enhanced proliferation and IL-2 production in Con A-stimulated splenocytes.
- It increased Th2 cytokine IL-4 production and expression.
- It decreased Th1 cytokine IFN-gamma production and expression.
Conclusions:
- Ginsenoside-Ro exhibits immunomodulatory activity.
- It regulates Th1/Th2 cytokine balance in murine splenocytes.
Aim:
To study the effects of ginsenoside-Ro on cell proliferation and cytokine production in murine splenocytes.
Methods:
The effect of ginsenoside-Ro on murine splenocytes proliferation was studied using [3H] thymidine incorporation assay. Effects of ginsenoside-Ro on the production of cytokines interleukin-2 (IL-2), interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) from murine splenocytes were detected by ELISA method. Effects of ginsenoside-Ro on mRNA level of Th1 cytokine IFN-gamma and Th2 cytokine IL-4 were evaluated by reverse transcription polymerase chain reaction (RT-PCR) analysis.
Results:
Ginsenoside-Ro showed no mitogenic effect on unstimulated murine splenocytes. It enhanced the proliferation of Con A-induced murine splenocytes and the production of IL-2 at concentrations of 1-10 micromol x L(-1). Moreover, ginsenoside-Ro increased the production and expression of Th2 cytokine IL-4 and decreased the production and expression of Th1 cytokine IFN-gamma in Con A-induced murine splenocytes at concentrations of 2-10 micromol x L(-1).
Conclusion:
Ginsenoside-Ro showed immunomodulatory effects by regulating the production and expression of Th1/Th2 cytokines in murine splenocytes.
