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A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3
11:44

A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3

Published on: January 24, 2016

IRF-3 releases its inhibitions

John Hiscott, Rongtuan Lin

    Structure (London, England : 1993)
    |September 13, 2005
    PubMed

    Insights

    Interferon regulatory factor (IRF) 3 activates antiviral genes. Its structure with CBP/p300 coactivator reveals IRF activation mechanisms and CBP/p300 flexibility.

    Area of Science:

    • Molecular Biology
    • Structural Biology
    • Immunology

    Background:

    • Interferon regulatory factor (IRF) 3 is a key transcription factor.
    • IRF-3 activation initiates the expression of interferon-stimulated genes (ISGs) crucial for antiviral defense.

    Discussion:

    • The study presents the structure of IRF-3 bound to the CBP/p300 coactivator.
    • This structural information elucidates the molecular mechanisms underlying IRF-3 activation.
    • It also highlights the inherent structural flexibilities of the CBP/p300 coactivator.

    Key Insights:

    • Reveals the structural basis for IRF-3 mediated transcriptional activation.
    • Demonstrates how CBP/p300 accommodates conformational changes during coactivation.
    • Provides insights into the dynamic interplay between transcription factors and coactivators.

    Outlook:

    • Potential for developing novel therapeutics targeting IRF-3 or CBP/p300 pathways.
    • Further structural studies of related transcription factor complexes.
    • Understanding these mechanisms aids in modulating innate immune responses.

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