Related Experiment Video
Updated: Aug 8, 2026
Evaluation of Tumor-infiltrating Leukocyte Subsets in a Subcutaneous Tumor Model
Published on: April 13, 2015
Eighth Leucocyte Differentiation Antigen Workshop DC section summary
Georgina Clark1, David Munster, Shakeel Yusuf
1Dendritic Cell Laboratory, Mater Medical Research Institute, Aubigny Place, Mater Health Services, South Brisbane Q4101, Australia. gclark@mmri.mater.org.au
Insights
Dendritic cells (DCs) are key immune cells. The eighth Human Leucocyte Differentiation Antigen workshop characterized DC surface molecules, identifying eleven new CD markers for these antigen-presenting cells.
Area of Science:
- Immunology
- Cell Biology
Background:
- Dendritic cells (DCs) are crucial antigen-presenting cells initiating immune responses.
- DCs were recognized as a distinct leukocyte lineage at the 7th Human Leucocyte Differentiation Antigen workshop.
- Research increasingly focuses on in vitro differentiated and ex vivo purified DC populations.
Purpose of the Study:
- To assess surface molecule expression on various dendritic cell populations.
- To summarize findings from the dendritic cell section of the 8th Human Leucocyte Differentiation Antigen workshop (HLDA8).
Main Methods:
- Evaluation of leukocyte surface molecule expression on diverse DC preparations.
- Utilizing monoclonal antibodies and molecular data to identify cell surface molecules.
Main Results:
- Detailed summary of molecule expression across different DC populations.
- Identification of significant differences between various DC preparations.
- Allocation of eleven new CD (Cluster of Differentiation) markers based on identified molecules.
Conclusions:
- The HLDA8 workshop provided critical insights into dendritic cell surface markers.
- New CD designations enhance the characterization and study of dendritic cells.
- This work contributes to understanding the heterogeneity and identification of dendritic cell subsets.
Abstract:
Dendritic cells (DC) are specialist antigen presenting cells that play a role in the initiation of innate and adaptive immune response. At the seventh Human Leucocyte Differentiation Antigen workshop, these intriguing cell populations were included as a separate lineage of leucocytes. This paper reports the studies performed in the eighth Human Leucocyte Differentiation Antigen workshop as part of the DC section. Many investigators currently focus on DC that are derived from a number of different leucocyte populations, including those that are differentiated in vitro and cells that are purified ex vivo. The DC section assessed the surface expression of different leucocyte surface molecules on a range of different DC populations. The results summarise the expression of each molecule on dendritic cell populations and differences between different DC preparations. Eleven new CDs were allocated on the basis of monoclonal antibodies and molecular information that identify known cell surface molecules expressed by dendritic cells. This paper gives a brief review of the work that was performed during the HLDA8 and a summary of the CDs represented by submitted mAb.
