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Updated: Aug 8, 2026
Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis
Published on: January 12, 2015
[Human adipose derived stem cells suppress lymphocyte proliferation induced by cellular or nonspecific mitogenic
1Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Second Medical University, Shanghai Tissue Engineering Research and Development Center, Shanghai 200011, China.
Insights
Adipose-derived stem cells (ADSC) do not trigger immune responses and can modulate lymphocyte activity in vitro. These findings highlight ADSC
Area of Science:
- Immunology
- Stem Cell Biology
Context:
- Adipose-derived stem cells (ADSC) are being investigated for their therapeutic potential.
- Understanding the immunomodulatory properties of ADSC is crucial for their clinical application.
Purpose:
- To investigate the immunological characteristics of ADSC.
- To evaluate the in vitro immunomodulatory effects of ADSC on lymphocytes.
Summary:
- ADSC were isolated and analyzed for Human Leukocyte Antigen (HLA) expression.
- Peripheral blood lymphocytes (PBL) were cultured with ADSC in mixed lymphocyte reactions (MLR).
- ADSC demonstrated dose-dependent inhibition of lymphocyte proliferation, even after IFN-gamma treatment.
Impact:
- Undifferentiated ADSC do not induce alloreactive lymphocyte proliferation.
- ADSC possess immunomodulatory capabilities, suggesting potential for immune tolerance induction.
- These findings support the use of ADSC in cell-based therapies requiring immune modulation.
Objective:
To study immunological properties of adipose derived stem cells (ADSC) and their in vitro immunomodulatory effects on lymphocytes.
Methods:
ADSC was isolated from fat tissue by liposuction and culture expanded. Cells at passage 2 were observed for the expression of HLAI, HLAII by FACs analysis. Peripheral blood lymphocytes (PBL) were cultured with allogeneic ADSCs at dose of 1 x 10(5). A third-party ADSCs at dose of 1 x 10(2), 1 x 10(3), 1 x 10(4), 1 x 10(5) were added to the ongoing two-way mixed lymphocyte reactions (MLR) for 6 days. Value of CPM (count per minute) was calculated with luminescence counter.
Results:
Flow cytometry showed that undifferentiated ADSCs express HLA class I but not class II. Addition of interferon gamma (IFN-gamma) for 48 hours induced greater than 90% of cells to express HLA class II. No lymphocyte response was induced by allogeneic ADSCs as stimulators. Results were similar using ADSCs pretreated with IFN-gamma. ADSCs elicited inhibit function of mixed lymphocyte cultures in a dose dependent way. Even if ADSCs were pretreated with IFN-gamma, the suppression ability was maintained.
Conclusion:
Undifferentiated ADSCs do not elicit alloreactive lymphocyte proliferative responses and could modulate immune responses in vitro.
