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Published on: September 16, 2018
[Effects of Angelica sinensis polysaccharide on cell-mediated immunity]
Tie-hong Yang1, Min Jia, Qi-bing Mei
1Department of Pharmacology, Fourth Military Medical University, Xi'an 710032, China. yangtiehong@sina.com
Insights
Angelica sinensis polysaccharide (AP) enhances cell-mediated immunity by stimulating splenocyte and T lymphocyte proliferation. Specifically, AP-3 fraction boosts CD4(+) T lymphocyte percentages, indicating a targeted immune-modulating effect.
Area of Science:
- Immunology
- Pharmacology
- Natural Products
Context:
- Cell-mediated immunity plays a crucial role in host defense against pathogens and tumor surveillance.
- Angelica sinensis is a traditional Chinese herb with a long history of use in various medicinal applications.
- Understanding the immunomodulatory properties of plant-derived polysaccharides is essential for developing novel therapeutic agents.
Purpose:
- To investigate the immunomodulatory effects of Angelica sinensis polysaccharide (AP) on cell-mediated immunity.
- To determine the specific fractions of AP that exhibit significant immune-enhancing activities.
- To elucidate the impact of AP on T lymphocyte subsets, particularly CD4(+) T lymphocytes.
Summary:
- Angelica sinensis polysaccharide (AP) and its fractions (AP-0, AP-1, AP-2, AP-3) were tested for their effects on murine splenocyte proliferation.
- AP-3 significantly enhanced lymphocyte proliferation in mixed lymphocyte reactions (MLR) and T lymphocyte assays.
- Flow cytometry analysis revealed that AP-3 increased the percentage of CD4(+) T lymphocytes within total splenocytes.
Impact:
- AP demonstrates potential as an immunomodulatory agent, promoting cell-mediated immunity.
- The findings suggest that AP, particularly the AP-3 fraction, could be a valuable component in therapies aimed at boosting immune responses.
- Further research into AP's mechanisms of action could lead to the development of new immunotherapeutic strategies.
Aim:
To investigate the effects of Angelica sinensis polysaccharide (AP) on cell-mediated immunity.
Methods:
The lymphocyte proliferation was tested by MTT colormetry; T lymphocyte fraction were separated with immune magnetic beads and the change of CD4(+) T lymphocyte percentage was detected by flow cytometry.
Results:
In the range of 30-300 mg/L, the total AP and its three fractions, ie AP-0, AP-1, AP-2, AP-3, directly stimulated the proliferation of murine splenocytes, and AP-3 significantly enhanced the proliferation of lymphocytes in MLR and T lymphocytes. The percentage of CD4(+) T lymphocytes in total splenocytes was also enhanced by AP-3.
Conclusion:
AP can promote the cell-mediated immunity with T lymphocytes as one of its target cells.
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