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Interrelationship between insulin-like growth factor I-induced activation of the Na+/H(+)-antiporter and

N Takasu1, I Komiya, Y Nagasawa

  • 1Department of Gerontology, Endocrinology and Metabolism, School of Medicine, Shinshu University, Nagano-ken, Japan.

Biochemical and Biophysical Research Communications
|May 31, 1991
PubMed

Insights

Insulin-like growth factor I (IGF-I) increases intracellular pH (pHi) and calcium in thyroid cells by activating the Na+/H+-antiporter. This pH shift optimizes the calcium response, a key biological function of IGF-I.

Area of Science:

  • Cellular Biology
  • Endocrinology

Background:

  • Insulin-like growth factor I (IGF-I) plays a role in cellular processes.
  • Intracellular pH (pHi) and calcium levels ([Ca2+]i) are critical for cell function.

Purpose of the Study:

  • To investigate the role of the Na+/H+-antiporter in IGF-I-induced changes in pHi and [Ca2+]i in porcine thyroid cells.
  • To elucidate the relationship between pHi and the [Ca2+]i response to IGF-I.

Main Methods:

  • Cultured porcine thyroid cells were treated with IGF-I.
  • The Na+/H+-antiporter was inhibited using dimethylamiloride or by reducing external Na+ concentrations.
  • Changes in pHi and [Ca2+]i were measured.

Main Results:

  • IGF-I significantly increased both pHi and [Ca2+]i in thyroid cells.
  • Inhibition of the Na+/H+-antiporter attenuated the IGF-I-induced increases in pHi and [Ca2+]i.
  • The [Ca2+]i response to IGF-I was found to be dependent on the resulting pHi.
  • IGF-I activates the Na+/H+-antiporter, leading to cellular alkalinization.

Conclusions:

  • IGF-I activates the Na+/H+-antiporter, increasing pHi in porcine thyroid cells.
  • The observed increase in pHi facilitates the calcium response to IGF-I by optimizing the intracellular Ca2+-mobilizing system.
  • Activation of the Na+/H+-antiporter by IGF-I is crucial for modulating intracellular calcium levels.

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