Related Experiment Video

Updated: Oct 3, 2025

Histological-Based Stainings Using Free-Floating Tissue Sections
06:45

Histological-Based Stainings Using Free-Floating Tissue Sections

Published on: August 25, 2020

19.6K

Immunohistochemistry

Cheryl E Gillett1

  • 1Hedley Atkins/Cancer Research UK Breast Pathology Laboratory, Guy's Hospital, London.

Methods in Molecular Medicine
|February 24, 2006
PubMed

Insights

Immunohistochemistry techniques enhance protein visualization in tissues. Reliable control materials are crucial for optimizing and monitoring novel breast-associated antibody performance and specificity.

Area of Science:

  • Biochemistry
  • Immunology
  • Histology

Background:

  • Immunohistochemistry (IHC) visualizes specific proteins within cellular or tissue structures.
  • IHC sensitivity has advanced through labeled polymer systems, enabling detection of low-level antigens.
  • Ensuring specificity is critical alongside antigen recovery and signal amplification in IHC.

Purpose of the Study:

  • To highlight the importance of specificity in immunohistochemistry.
  • To emphasize the role of control materials in optimizing IHC assays.
  • To guide the use of novel breast-associated antibodies.

Main Methods:

  • Review of immunohistochemistry techniques and advancements.
  • Discussion of antigen recovery and signal amplification strategies.
  • Emphasis on the use of control materials for antibody validation.

Main Results:

  • Improved sensitivity in IHC allows detection of low antigen levels.
  • Specificity must be rigorously demonstrated for reliable IHC results.
  • Control materials are essential for validating antibody performance.

Conclusions:

  • Optimizing IHC requires careful selection of methods for antigen recovery and signal amplification.
  • The use of pertinent and reliable control material is indispensable for validating novel breast-associated antibodies.
  • Ensuring specificity is paramount for accurate protein detection in tissue samples.

More Related Videos

Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections09:11

Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections

Published on: November 14, 2016

17.3K
Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging08:40

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

Published on: April 8, 2016

13.0K

Related Concept Videos

Immunocytochemistry and Immunohistochemistry01:22

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...
12.3K
Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
4.5K
Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
11.6K