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CD147 contains different bioactive epitopes involving the regulation of cell adhesion and lymphocyte activation

Sawitree Chiampanichayakul1, Pakorn Peng-in, Panida Khunkaewla

  • 1Clinical Microscopy Branch, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.

Immunobiology
|March 15, 2006
PubMed

Insights

This study generated five CD147 monoclonal antibodies to investigate CD147 functions. Certain antibodies inhibited lymphocyte proliferation, while others induced cell aggregation, revealing distinct bioactive domains on CD147.

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • CD147 is a leukocyte surface molecule within the immunoglobulin superfamily.
  • It is widely expressed and associated with lymphocyte activation.
  • Understanding CD147's function is crucial for immunological research.

Purpose of the Study:

  • To generate and characterize CD147-specific monoclonal antibodies (mAbs).
  • To investigate the functional roles of different CD147 domains.
  • To elucidate the relationship between CD147 epitopes and biological activities.

Main Methods:

  • Generation of five CD147 mAbs (M6-2F9, M6-1D4, M6-1F3, M6-1B9, M6-1E9).
  • Biochemical characterization and cross-blocking experiments.
  • Functional assays including lymphocyte proliferation inhibition and U937 cell aggregation induction using COS transfectants expressing CD147 domains.

Main Results:

  • mAbs M6-1B9 and M6-1E9 recognize the same or adjacent epitopes on CD147.
  • mAbs M6-2F9, M6-1D4, M6-1B9, and M6-1E9 bind to domain 1 of CD147.
  • mAbs M6-1B9 and M6-1E9 inhibited CD3 mAb-induced lymphocyte proliferation.
  • mAbs M6-2F9, M6-1D4, and M6-1F3 induced U937 homotypic cell aggregation.

Conclusions:

  • CD147 possesses at least two distinct bioactive domains.
  • Epitopes involved in cell aggregation differ from those regulating lymphocyte activation.
  • This research provides insights into CD147's multifaceted roles in immune responses.

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