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Ex Vivo Infection of Murine Epidermis with Herpes Simplex Virus Type 1
11:56

Ex Vivo Infection of Murine Epidermis with Herpes Simplex Virus Type 1

Published on: August 24, 2015

Biochemical characterization of herpes simplex virus type-1-immunostimulating complexes (ISCMOs): a

M Erturk1, R Jennings, R J Phillpotts

  • 1Department of Virology, University of Sheffield Medical School, UK.

Vaccine
|September 1, 1991
PubMed

Insights

Researchers developed an immunostimulating complex (ISCOM) vaccine containing multiple herpes simplex virus type 1 (HSV-1) glycoproteins. This HSV-1 ISCOM preparation shows potential for human vaccination against HSV-1 infections.

Area of Science:

  • Virology
  • Immunology
  • Vaccine Development

Background:

  • Herpes simplex virus type 1 (HSV-1) poses significant public health challenges.
  • Effective vaccines against HSV-1 are critically needed.

Purpose of the Study:

  • To prepare and characterize an immunostimulating complex (ISCOM) containing multiple HSV-1 glycoproteins.
  • To evaluate the potential of this HSV-1 ISCOM preparation as a human vaccine.

Main Methods:

  • Extraction of HSV-1 antigens using zwitterionic detergent.
  • Purification via gradient centrifugation.
  • Characterization using enzyme-linked immunosorbent assay (ELISA), SDS-PAGE, and radioimmunoprecipitation.
  • DNA content determination using 3H-thymidine labeling.

Main Results:

  • The HSV-1 ISCOM preparation contained multiple HSV-1 glycoproteins (B, C, D, E, H, I) and other proteins.
  • The protein and DNA content of the ISCOMs were quantified.

Conclusions:

  • The characterized HSV-1 ISCOM preparation contains key HSV-1 glycoproteins.
  • This preparation holds promise as a potential vaccine candidate for human use against HSV-1.

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