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Updated: Aug 7, 2026

Flow Cytometric Characterization of Murine B Cell Development
08:25

Flow Cytometric Characterization of Murine B Cell Development

Published on: January 22, 2021

B cells in murine cervical lymph nodes are conventional B-2 cells

Seung Geun Yeo1, Joong Saeng Cho, Dong Choon Park

  • 1Department of Otolaryngology, College of Medicine, Kyung Hee University, Seoul, Korea.

Journal of Korean Medical Science
|June 17, 2006
PubMed

Insights

B cells in mouse cervical lymph nodes exhibit conventional B2 cell characteristics, similar to splenic B cells, despite potential continuous antigen exposure. Their surface molecule expression and immunoglobulin secretion patterns align with those of splenic B cells.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Cervical lymph nodes receive antigens from the upper respiratory tract, potentially influencing local B cell behavior.
  • Understanding B cell heterogeneity is crucial for immune response characterization.

Purpose of the Study:

  • To investigate the distinct features of B cells in cervical lymph nodes compared to other lymphoid sites.
  • To determine if cervical lymph node B cells exhibit unique phenotypic or functional characteristics.

Main Methods:

  • Isolation of B cells from mouse cervical lymph nodes, spleen, and peritoneal fluid.
  • Flow cytometry to analyze surface molecule expression (CD5, CD23, CD43, B7.1, B7.2, Syndecan-1).
  • Enzyme-linked immunosorbent assay (ELISA) for immunoglobulin secretion and proliferative assays for cell cycle entry (S phase).

Main Results:

  • Cervical lymph node B cells displayed a phenotype of CD5 low, CD23high, CD43low, B7.1low, B7.2low, and Syndecan-1low.
  • Stimulated B cells showed increased IgM secretion over IgA and IgG, with active entry into S phase after 48 hours.
  • No significant differences were observed in the fundamental characteristics compared to splenic B cells.

Conclusions:

  • B cells within the cervical lymph nodes of mice are identified as conventional B2 cells.
  • Their phenotype and response to stimulation are comparable to B cells found in the spleen.

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