[Detection of the labile iron pool in leukemia cells and its significance]
Guo-Cun Jia1, Ju Gao, Qing-Kui Liao
1Department of Pediatric Hematology, Zhengzhou Children Hospital, Zhengzhou 450053, China.
Insights
Desferrioxamine (DFO) effectively reduces labile iron pool (LIP) in leukemia cells. This study presents a simple method for detecting intracellular iron homeostasis, crucial for cell health.
Area of Science:
- Cell Biology
- Biochemistry
- Hematology
Context:
- Labile iron pool (LIP) is critical for cellular function.
- Accurate detection of LIP is essential for understanding iron homeostasis.
- Leukemia cells exhibit altered iron metabolism.
Purpose:
- To develop a rapid and straightforward method for detecting intracellular LIP.
- To investigate the effect of desferrioxamine (DFO) on LIP in leukemia cells.
- To assess the utility of calcein-AM fluorescence for monitoring iron levels.
Summary:
- HL-60 and K562 leukemia cells were treated with varying concentrations of DFO to induce iron deprivation.
- Intracellular LIP was measured using the calcein-AM fluorescent probe.
- DFO treatment resulted in significantly lower calcein fluorescence, indicating reduced LIP compared to controls.
Impact:
- The DFO-induced reduction in LIP was observed in both HL-60 and K562 cells.
- The calcein-AM assay provides a simple and reliable approach for assessing intracellular iron homeostasis.
- This method can aid in understanding iron dysregulation in leukemia and potentially guide therapeutic strategies.
Abstract:
To explore a rapid and easy method to detect labile iron of pool (LIP) in cells, HL-60 and K562 cells were cultured at a concentration 1 x 10(6)/ml in RPMI 1640 containing 10% heat-inactivated fetal bovine serum. The iron deprivation was induced by adding desferrioxamine (DFO) 10 - 100 micromol/L for 0 - 48 hours. The intracellular LIP was measured by probe calcein-AM. Calcein fluorescence was monitored in 1420 multilabel counter. The results indicated that when HL-60 and K562 cells were incubated with different concentrations of DFO, the calcein fluorescence intensity was higher than that of control group at 12, 24 and 48 hours (P < 0.05). Fluorescence value of representing LIP in DFO groups was lower than that in the control group. In conclusion, DFO can decrease LIP in leukemia cells. The approach used in this study may provide a simple and reliable method for detection of intracellular iron homeostasis.
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