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Dynamic Adhesion Assay for the Functional Analysis of Anti-adhesion Therapies in Inflammatory Bowel Disease
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Expression, function and regulation of the intercellular adhesion molecule-1 (ICAM-1) on human intestinal epithelial

D Kaiserlian1, D Rigal, J Abello

  • 1INSERM U 80, CNRS URA 1177 UCBL, Hôpital E. Herriot, Lyon, France.

European Journal of Immunology
|October 1, 1991
PubMed

Insights

Human intestinal cells express intercellular adhesion molecule-1 (ICAM-1), a rhinovirus receptor. Its expression and regulation by cytokines suggest a role in immune surveillance of colon cancer and inflammatory gut diseases.

Area of Science:

  • Immunology
  • Gastroenterology
  • Molecular Biology

Background:

  • Intercellular Adhesion Molecule-1 (ICAM-1) is crucial for immune cell interactions.
  • Its role in intestinal adenocarcinoma and inflammatory gut diseases requires further elucidation.

Purpose of the Study:

  • To characterize ICAM-1 expression on human intestinal adenocarcinoma cell lines.
  • To investigate the regulation of ICAM-1 by cytokines and its functional role in T cell adhesion.

Main Methods:

  • Characterization of ICAM-1 as a 93 kDa polypeptide using biochemical methods.
  • Assessment of ICAM-1 expression levels in relation to enterocytic differentiation.
  • Investigation of ICAM-1 upregulation by phorbol ester and cytokines (IFN-γ, IL-1β).
  • Analysis of T cell adhesion to enterocytes mediated by ICAM-1/LFA-1 interaction.

Main Results:

  • ICAM-1 was identified as a 93 kDa protein and a rhinovirus receptor on intestinal adenocarcinoma cells.
  • ICAM-1 expression correlated positively with enterocytic differentiation, being highest in Caco-2 cells.
  • Less differentiated cell lines (HT29, T84) showed ICAM-1 upregulation by PMA, IFN-γ, and IL-1β.
  • Enterocyte ICAM-1 mediated adhesion to activated T cells via leukocyte function-associated antigen-1 (LFA-1).

Conclusions:

  • Colon adenocarcinoma cell lines express functional ICAM-1 that is regulated by cytokines.
  • The ICAM-1/LFA-1 pathway may play a role in the immune surveillance of colon adenocarcinomas.
  • This pathway could also be implicated in inflammatory bowel disease and celiac disease pathogenesis.

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