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The bovine p150/95 molecule (CD11c/CD18) functions in primary cell-cell interaction

L Eskra1, K L O'Reilly, G A Splitter

  • 1Department of Veterinary Science, University of Wisconsin-Madison 53706.

Veterinary Immunology and Immunopathology
|September 1, 1991
PubMed

Insights

The monoclonal antibody C5B6 targets the CD11c/CD18 molecule on bovine monocytes, inhibiting primary immune responses to alloantigens. This antibody shows specificity for certain bovine lymphocytes but not other species.

Area of Science:

  • Immunology
  • Veterinary Science
  • Cell Biology

Background:

  • Monoclonal antibodies (MAbs) are crucial tools for identifying cell surface molecules.
  • The CD11c/CD18 molecule is a key component of the leukocyte adhesion receptor family.
  • Understanding bovine immune cell markers aids in diagnosing and treating animal diseases.

Purpose of the Study:

  • To characterize the specificity and function of the monoclonal antibody C5B6.
  • To investigate the role of the CD11c/CD18 molecule in bovine immune cells.
  • To evaluate the potential of C5B6 as an immunomodulatory agent in cattle.

Main Methods:

  • Flow cytometry was used to determine MAb C5B6 reactivity with bovine peripheral blood leukocytes.
  • Immunoprecipitation assays were performed to identify the molecular weight of antigen recognized by C5B6.
  • Functional assays assessed the effect of C5B6 on lymphocyte proliferation and cytotoxicity.

Main Results:

  • MAb C5B6 specifically recognized the CD11c/CD18 molecule on bovine monocytes and granulocytes.
  • Immunoprecipitation revealed bands of 150,000 and 95,000 Da, consistent with the CD11c/CD18 complex.
  • C5B6 inhibited the primary but not secondary proliferative response to alloantigens, without affecting cytotoxicity.

Conclusions:

  • MAb C5B6 is a valuable reagent for identifying bovine monocytes and granulocytes expressing CD11c/CD18.
  • The CD11c/CD18 molecule plays a role in the primary immune response to alloantigens in cattle.
  • C5B6 demonstrates potential for modulating specific immune responses in bovine applications.

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