Related Experiment Video
Updated: Aug 8, 2026
Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses
Published on: November 16, 2016
Interferon alpha-induced modulation of leukocyte cell surface antigens: immunocytofluorometric study with human
B Chorváth1, J Sedlák, N Fuchsberger
1Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Czechoslovakia.
Insights
Recombinant interferon alpha increases MHC class I antigen density on leukemia and lymphoma cell lines. However, it did not significantly alter other key cell surface markers like CD45 or CD71.
Area of Science:
- Immunology
- Cell Biology
- Oncology
Background:
- Interferons are crucial in immune response and cellular regulation.
- Understanding interferon's effects on cancer cell surface antigens is vital for therapeutic development.
- Leukemia and lymphoma cell lines serve as models for studying these interactions.
Purpose of the Study:
- To investigate the impact of recombinant interferon alpha on the expression of major histocompatibility complex (MHC) class I antigens.
- To evaluate the effects of interferon alpha on other cell surface markers in human leukemia and lymphoma cell lines.
- To compare the effects of recombinant interferon alpha with human leukocyte interferon alpha.
Main Methods:
- Utilized immunocytofluorometry with specific monoclonal antibodies.
- Analyzed human leukemia/lymphoma cell lines (REH, U-937, HL-60, K-562).
- Assessed expression of MHC class I, MHC class II, CD45, CD71, CD10, and CD4 antigens.
Main Results:
- Recombinant interferon alpha significantly enhanced MHC class I antigen density on REH, U-937, and HL-60 cells.
- Human leukocyte interferon alpha showed a similar effect on MHC class I in REH cells.
- No major alterations in CD45 or CD71 expression were observed; CD10 remained unchanged in REH cells. CD4 density decreased on U-937 cells. K-562 cells showed no induction of MHC antigens.
Conclusions:
- Interferon alpha effectively upregulates MHC class I antigens on specific leukemia/lymphoma cell lines.
- The effects on other cell surface markers are variable and cell-type dependent.
- These findings contribute to understanding interferon's immunomodulatory role in hematological malignancies.
Abstract:
Recombinant interferon alpha enhanced the MHC class I antigen density on human leukaemia/lymphoma cell lines REH, U-937 and HL-60, as measured by immunocytofluorometry using specific monoclonal antibodies. A similar effect was induced (as demonstrated in REH cells), also by human leukocyte interferon-alpha. The latter, however, caused no major alterations in the expression of leukocyte common antigen (ICA; CD45) and transferrin receptor (CD71) in the cell lines examined. In REH cells, there was no interferon-induced alteration of CD10 antigen (CALLA), which in this cell line is markedly down-regulated by 12-0-tetradecanoyl-phorbol-13-acetate (TPA). A decrease of CD4 antigen density on the cell membrane was induced by interferon-alpha in monoblastoid U-937 cells. No induction of MHC class I and II antigens by interferon-alpha was found in K-562 cell subline.
More Related Videos
08:26Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α
Published on: June 14, 2018
09:11Imaging of In Situ Interferon Gamma Production in the Mouse Spleen following Listeria monocytogenes Infection
Published on: July 16, 2019