Related Experiment Video

Updated: Aug 7, 2026

Development and Functional Characterization of Murine Tolerogenic Dendritic Cells
09:51

Development and Functional Characterization of Murine Tolerogenic Dendritic Cells

Published on: May 18, 2018

CD45 is required for type I IFN production by dendritic cells

Maria Montoya1, Ritu Dawes, Delyth Reid

  • 1The Edward Jenner Institute for Vaccine Research, Compton, UK.

European Journal of Immunology
|July 21, 2006
PubMed

Insights

CD45 deficiency in dendritic cells (DCs) increases plasmacytoid DC numbers and impairs type I interferon production during lymphocytic choriomeningitis virus (LCMV) infection. CD45RABC and CD45RO transgenes largely restore DC function.

Area of Science:

  • Immunology
  • Cell Biology
  • Virology

Background:

  • CD45, a leukocyte tyrosine phosphatase, is crucial for immune responses.
  • Dendritic cells (DCs) are key antigen-presenting cells in immunity.
  • The role of CD45 in DC function during viral infections is not fully understood.

Purpose of the Study:

  • To investigate the function of splenic dendritic cells lacking CD45 expression.
  • To determine the impact of CD45 deficiency on DC response to lymphocytic choriomeningitis virus (LCMV) infection.
  • To evaluate the restorative effects of CD45RABC and CD45RO transgenes on CD45-deficient DCs.

Main Methods:

  • Comparative analysis of splenic dendritic cells from wild-type (CD45+/+), CD45-deficient (CD45-/-), and transgenic (CD45RABC, CD45RO) mice.
  • Assessment of dendritic cell numbers, including plasmacytoid dendritic cells.
  • Evaluation of dendritic cell response to LCMV infection, including MHC and costimulatory molecule expression.
  • Measurement of type I interferon production by dendritic cells in response to LCMV infection in vivo.

Main Results:

  • CD45-/- mice exhibited increased numbers of plasmacytoid dendritic cells.
  • Dendritic cells from all mouse groups showed upregulation of MHC and costimulatory molecules upon LCMV infection.
  • Dendritic cells from CD45-/- mice demonstrated impaired type I interferon production following LCMV infection in vivo.
  • CD45RABC and CD45RO transgenes largely restored the function of CD45-deficient dendritic cells.

Conclusions:

  • Lack of CD45 expression in dendritic cells significantly affects their function, particularly in type I interferon production during viral infections.
  • CD45 plays a critical role in regulating dendritic cell responses to LCMV infection.
  • CD45RABC and CD45RO transgenes can rescue the impaired function of CD45-deficient dendritic cells, highlighting the importance of specific CD45 isoforms.

More Related Videos

An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets09:09

An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets

Published on: April 18, 2016

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro06:12

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro

Published on: March 7, 2022

Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Type I Diabetes II: Pathophysiology01:26

Type I Diabetes II: Pathophysiology

Type 1 diabetes mellitus arises from an immune-mediated destruction of pancreatic β-cells, resulting in an absolute deficiency of insulin. This process develops in genetically susceptible individuals when autoimmunity, environmental exposures, and immunologic dysregulation converge to trigger a targeted attack on the insulin-producing cells of the pancreas. The β-cells are located within the islets of Langerhans and are essential for regulating blood glucose by facilitating cellular uptake of...