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Visualizing Antigen Specific CD4+ T Cells using MHC Class II Tetramers
15:42

Visualizing Antigen Specific CD4+ T Cells using MHC Class II Tetramers

Published on: March 6, 2009

Human memory T cells express intercellular adhesion molecule-1 which can be increased by interleukin 2 and

A M Buckle1, N Hogg

  • 1Macrophage Laboratory, Imperial Cancer Research Fund, Lincoln's Inn Fields, London.

European Journal of Immunology
|February 1, 1990
PubMed

Insights

Memory T cells express intercellular adhesion molecule-1 (ICAM-1), enabling rapid recall antigen responses. ICAM-1 expression on T cells is upregulated by cytokines and blocking it inhibits antigen response, indicating its functional role.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Intercellular adhesion molecule-1 (ICAM-1) is expressed on T cells.
  • Memory T cells possess distinct phenotypic and functional characteristics compared to naive T cells.

Purpose of the Study:

  • To investigate the expression and function of ICAM-1 on peripheral blood T cells.
  • To determine the role of ICAM-1 in T cell activation and response to recall antigens.

Main Methods:

  • Flow cytometry to detect ICAM-1 expression on T cells.
  • Functional assays to assess T cell response to recall antigens.
  • Cytokine stimulation assays to evaluate ICAM-1 upregulation.

Main Results:

  • Approximately 40% of resting peripheral blood T cells express ICAM-1.
  • ICAM-1+ T cells exhibit memory cell markers and respond rapidly to recall antigens.
  • Interleukin-2 and interferon-gamma increase ICAM-1 expression on T cells.
  • Anti-ICAM-1 antibody treatment inhibits T cell response to recall antigens.

Conclusions:

  • ICAM-1 is a functional marker on memory T cells involved in recall antigen responses.
  • Cytokines regulate ICAM-1 expression, suggesting a role in T cell activation.
  • ICAM-1 plays a significant role in the functional capabilities of memory T cells.

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