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Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture
10:31

Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture

Published on: April 8, 2016

Subsets of keratinocytes and Langerhans' cells express epitopes associated with suppressor-inducer capabilities in

G De Panfilis1, G C Manara, C Ferrari

  • 1Department of Dermatology, University of Parma, Italy.

Immunology
|April 1, 1990
PubMed

Insights

Human epidermis contains two cell types, keratinocytes and Langerhans

Area of Science:

  • Immunology
  • Dermatology
  • Cell Biology

Background:

  • Murine epidermis harbors cells with immune-down-regulatory functions.
  • Identification of similar cell populations in human epidermis is crucial for understanding cutaneous immunity.

Purpose of the Study:

  • To investigate the presence and distribution of CD45R-positive cells in human epidermis.
  • To identify potential cell populations involved in immune down-regulation within the human skin.

Main Methods:

  • Utilized 5-nm colloidal gold-immunoelectron microscopy with anti-CD45R monoclonal antibodies.
  • Analyzed freshly isolated human epidermal cell suspensions.
  • Scrutinized approximately 4000 cells per sample using electron microscopy.

Main Results:

  • Over 2% of the total human epidermal cell population expressed CD45R.
  • CD45R positivity was observed in approximately 2% of keratinocytes and 5% of Langerhans' cells.
  • Heterogeneous staining confirmed the presence of distinct CD45R+ subpopulations.

Conclusions:

  • Human epidermis contains at least two CD45R-positive cell populations: keratinocytes and Langerhans' cells.
  • These CD45R+ cells are potential candidates for mediating immune down-regulation in human skin.
  • Further functional studies are required to elucidate their precise roles in cutaneous immune responses.

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