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Updated: Jul 20, 2026

Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma
07:52

Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma

Published on: January 9, 2019

[Flow fluorimetry in differential diagnosis of diffuse large B-cell lymphoma]

I A Vorob'ev, L A Gorgidze, E M Gretsov

    Terapevticheskii Arkhiv
    |September 2, 2006
    PubMed

    Insights

    Flow cytometry effectively identifies B-cell clonality and assesses tumor cell proliferation in diffuse large B-cell lymphoma (DLBCL). This method aids in precise diagnosis and understanding of DLBCL tumor biology.

    Area of Science:

    • Hematology
    • Immunology
    • Oncology

    Context:

    • Diffuse large B-cell lymphoma (DLBCL) is an aggressive non-Hodgkin lymphoma.
    • Accurate immunophenotyping is crucial for DLBCL diagnosis and subclassification.
    • Flow cytometry offers a rapid and sensitive method for cellular analysis.

    Purpose:

    • To analyze the immunophenotype of diffuse large B-cell lymphoma (DLBCL) using flow cytometry.
    • To evaluate the utility of flow cytometry in assessing B-cell clonality and proliferative potential in DLBCL.

    Summary:

    • Flow cytometry analysis of DLBCL revealed phenotypes often resembling marginal zone lymphoma cells.
    • Studying cells with maximal light diffusion provided more precise clonality and proliferative potential assessment.
    • The proliferative index in 33 DLBCL cases ranged from 10-60%.

    Impact:

    • Flow cytometry enables reliable identification of B-cell clonality in most DLBCL cases.
    • This technique allows for a more precise evaluation of the tumor's proliferative capacity.
    • Enhanced diagnostic capabilities for DLBCL management and research.
    Abstract

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