Related Experiment Video

Updated: Aug 13, 2026

HPLC-based Assay to Monitor Extracellular Nucleotide/Nucleoside Metabolism in Human Chronic Lymphocytic Leukemia Cells
11:29

HPLC-based Assay to Monitor Extracellular Nucleotide/Nucleoside Metabolism in Human Chronic Lymphocytic Leukemia Cells

Published on: July 20, 2016

Normal B cell compartment in chronic lymphatic leukemia

A Pluta1, A Górski, M Nowaczyk

  • 1Department of Immunology, Transplantation Institute, Warsaw Medical School, Poland.

Hematological Oncology
|May 1, 1990
PubMed

Insights

Chronic lymphocytic leukemia (CLL) cells express CD5 antigen, unlike normal B lymphocytes. Advanced CLL stages may show a decrease in normal B cells, though disease progression showed no clear correlation with studied parameters.

Area of Science:

  • Immunology
  • Hematology
  • Oncology

Background:

  • Chronic lymphocytic leukemia (CLL) is characterized by the accumulation of malignant B lymphocytes.
  • CLL cells uniquely express the CD5 antigen, typically associated with T cells, distinguishing them from normal B cells.

Purpose of the Study:

  • To investigate the expression of CD5 and DR antigens on both normal and malignant B cells in CLL patients.
  • To evaluate potential correlations between B cell compartment characteristics and disease progression.

Main Methods:

  • Utilized two-colour immunofluorescence techniques.
  • Employed CD5-TRITC and DR-FITC staining to analyze B cell populations.

Main Results:

  • No significant correlation was observed between disease progression and the studied immunological parameters.
  • A trend towards a reduction in normal B lymphocytes was noted in patients with advanced stages of CLL.

Conclusions:

  • The CD5 marker's presence on CLL cells remains a key diagnostic feature.
  • While not directly correlated with progression, a decline in normal B cells in advanced disease warrants further investigation.

More Related Videos

A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia09:52

A Chromatin Immunoprecipitation Assay to Identify Novel NFAT2 Target Genes in Chronic Lymphocytic Leukemia

Published on: December 4, 2018

Subcellular Fractionation of Primary Chronic Lymphocytic Leukemia Cells to Monitor Nuclear/Cytoplasmic Protein Trafficking11:39

Subcellular Fractionation of Primary Chronic Lymphocytic Leukemia Cells to Monitor Nuclear/Cytoplasmic Protein Trafficking

Published on: October 23, 2019

Related Concept Videos

Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
Structure and Function of Leukocytes01:21

Structure and Function of Leukocytes

An adult in good health typically has between 4,500 and 11,000 leukocytes, or white blood cells, per microliter of blood, which constitutes about 1% of the total blood volume. Unlike red blood cells, white blood cells contain a nucleus and other cellular organelles but do not have hemoglobin. Most white blood cells reside in connective tissues, particularly in lymphatic organs such as the lymph nodes, with only a small fraction present in circulating blood.
White blood cells protect the body...
Disorders of Leukocytes01:27

Disorders of Leukocytes

Leukocyte disorders can lead to either leukopenia, characterized by an abnormally low leukocyte count, or leukocytosis, marked by a very high leukocyte number.
Leukopenia may result from bone marrow disorders, autoimmune diseases, and infectious diseases. For example, conditions such as multiple myeloma and aplastic anemia can impair the bone marrow's ability to produce adequate leukocytes. Similarly, autoimmune diseases like lupus and viral infections such as HIV can prompt the immune system...
Lymphoid Cells and Tissues01:18

Lymphoid Cells and Tissues

Lymphoid cells and tissues are integral to the immune system, which is crucial in maintaining our body's defense against harmful pathogens. They form the building blocks of lymphoid organs, which include the spleen, thymus, and lymph nodes.
Lymphoid cells consist of various types of immune system cells. These include B and T lymphocytes, which are responsible for producing antibodies and killing infected cells, respectively. Dendritic cells act as messengers between the innate and adaptive...
Primary Lymphoid Organs01:16

Primary Lymphoid Organs

Primary lymphoid organs are pivotal in the formation, development, and maturation of lymphocytes, the white blood cells that serve as the backbone of our immune system. This crucial function underscores their fundamental role in maintaining our overall health and immunity. The two primary lymphoid organs of prime importance are the red bone marrow and the thymus.
The red bone marrow is a soft, spongy tissue nestled in the interior of long bones such as the humerus and femur. It is the site...