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Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes
09:14

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes

Published on: June 13, 2014

Vascular cell adhesion molecule-1 mediates lymphocyte adherence to cytokine-activated cultured human endothelial

T M Carlos1, B R Schwartz, N L Kovach

  • 1Department of Medicine, University of Washington, Seattle.

Blood
|September 1, 1990
PubMed

Insights

Vascular cell adhesion molecule-1 (VCAM-1) is a novel protein induced by cytokines on endothelial cells. It mediates lymphocyte adhesion to inflamed endothelium via a pathway independent of CD18.

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • Cytokine-induced cell adhesion molecules play critical roles in immune responses.
  • Understanding these interactions is key to modulating inflammatory processes.

Purpose of the Study:

  • To characterize the expression and function of vascular cell adhesion molecule-1 (VCAM-1).
  • To investigate the role of VCAM-1 in lymphocyte adherence to cytokine-stimulated endothelium.

Main Methods:

  • Enzyme-linked immunosorbent assay (ELISA) and radioimmunoprecipitation were used to assess VCAM-1 expression.
  • Monoclonal antibody (MoAb) 4B9 was employed to block VCAM-1 function.
  • Lymphocyte adherence assays were performed on cytokine-activated human umbilical vein endothelium (HUVE).

Main Results:

  • VCAM-1 expression was minimal on unstimulated HUVE but rapidly induced by tumor necrosis factor-alpha (TNF-alpha), interleukin-1, and lipopolysaccharide.
  • MoAb 4B9 significantly inhibited peripheral blood lymphocyte (PBL) adherence to activated HUVE.
  • VCAM-1 mediated a CD18-independent pathway for PBL adherence.

Conclusions:

  • VCAM-1 is a cytokine-inducible adhesion molecule on endothelium.
  • VCAM-1 facilitates lymphocyte binding to inflamed endothelium through a CD18-independent mechanism.
  • This interaction may be crucial for lymphocyte emigration during inflammation.

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