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Published on: May 4, 2012
Antibodies to cytoskeletal systems in normal human serum
1Department of Clinical Immunology, Medical Institute of Bioregulation, Kyushu University, Beppu.
Insights
This study found that immunoglobulin M (IgM) levels correlate with antibodies to vimentin and cytokeratin in children and adults. Antibody levels to actin increase with age in adults.
Area of Science:
- Immunology
- Cell Biology
- Biochemistry
Background:
- Cytoskeletal proteins like actin, vimentin, and cytokeratin are crucial cellular components.
- Serum antibodies against these cytoskeletal systems can be indicative of various physiological and pathological states.
- Understanding the prevalence and correlation of these antibodies in different age groups is important for immunological research.
Purpose of the Study:
- To investigate the presence and levels of serum antibodies targeting actin, vimentin, and cytokeratin.
- To explore the correlation between antibody titers and immunoglobulin (Ig) concentrations, particularly IgM, IgG, and IgA.
- To examine age-related differences in anti-cytoskeletal antibody profiles.
Main Methods:
- Indirect immunofluorescence was employed using human skin fibroblasts (for actin and vimentin) and HEp2 cells (for cytokeratin).
- Serum samples from umbilical cord blood, 8-year-old pupils, and healthy adults were analyzed.
- Quantification of immunoglobulin M (IgM), immunoglobulin G (IgG), and immunoglobulin A (IgA) concentrations and antibody titers.
Main Results:
- Antibodies to cytokeratin were detected in umbilical cord blood with IgM concentrations above 10 mg/dl.
- Elevated IgG, IgM, and IgA antibody titers to vimentin and IgG and IgM titers to cytokeratin were observed in 8-year-old pupils.
- Healthy adults exhibited IgM anti-vimentin and anti-cytokeratin antibodies at 1:60 dilution, with IgG anti-actin antibodies appearing with increasing age. IgM concentration correlated with IgM antibody titers to vimentin and cytokeratin, while IgG and IgA showed no correlation.
Conclusions:
- Serum IgM levels show a tendency to correlate with IgM antibody titers against vimentin and cytokeratin.
- Age is a factor in the development of anti-cytoskeletal antibodies, specifically IgG class antibodies to actin in adults.
- The study highlights age-dependent variations and IgM-related correlations in anti-cytoskeletal antibody responses.
Abstract:
Serum antibodies to cytoskeletal systems were measured by indirect immunofluorescence using human skin fibroblasts as the substrate for actin and vimentin, and HEp2 cells for cytokeratin. IgM concentration in 12 umbilical cord blood was less than 20 mg/dl, but antibody to cytokeratin was detected in samples that contained more than 10 mg/dl of IgM. Sera from 8-year-old pupils had elevated antibody titer to vimentin of IgG, IgM and IgA class and to cytokeratin of IgG and IgM class. Healthy adults had IgM anti-vimentin and anti-cytokeratin antibody at 60 times serum dilution, but other antibodies were lower than 10 times serum dilution. IgG and IgA concentration did not have any correlation with anti-cytoskeletal system antibodies. IgM concentration tended to correlate with IgM antibody titer to vimentin and cytokeratin. With increasing age in adults, antibody to actin of IgG class appeared.
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