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A Method for Mouse Pancreatic Islet Isolation and Intracellular cAMP Determination
Published on: June 25, 2014
Curcumin inhibits in vitro MCP-1 release from mouse pancreatic islets
M M Amoli1, R Mousavizadeh, R Sorouri
1Endocrinology and Metabolism Resaerch Centre, Tehran University of Medical Sciences, Tehran, Iran. mahsa_amoli@hotmail.com
Insights
Curcumin, an anti-inflammatory compound, significantly reduced monocyte chemoattractant protein-1 (MCP-1) release from mouse pancreatic islets. This suggests curcumin
Area of Science:
- Immunology
- Endocrinology
- Pharmacology
Background:
- Monocyte chemoattractant protein-1 (MCP-1) is a chemokine involved in inflammatory responses and graft rejection.
- MCP-1 release from pancreatic islets is regulated by the nuclear factor-kappaB (NF-kappaB) pathway.
- Curcumin is known to inhibit NF-kappaB and possesses anti-inflammatory properties.
Purpose of the Study:
- To investigate the effect of curcumin on the in vitro release of MCP-1 from pancreatic islets.
- To evaluate curcumin's potential as a therapeutic agent in conditions involving islet inflammation.
Main Methods:
- Mouse pancreatic islets were cultured and treated with varying concentrations of curcumin (0, 10, 20 micromol/L).
- Islets were also treated with lipopolysaccharide (LPS) to induce inflammation.
- MCP-1 levels in culture supernatants were quantified using an enzyme-linked immunosorbent assay (ELISA).
Main Results:
- A significant decrease in MCP-1 release was observed at 20 micromol/L curcumin compared to controls (P = .005).
- Curcumin treatment reduced MCP-1 levels in LPS-stimulated islets at both 10 and 20 micromol/L concentrations (P = .01).
Conclusions:
- Curcumin effectively inhibits MCP-1 release from pancreatic islets in vitro.
- These findings support curcumin's potential role in mitigating inflammatory processes associated with islet dysfunction and transplantation.
Objectives:
Monocyte chemoattractant proteins (MCP-1) belongs to the CC family of chemokines secreted from islets of the pancreas, producing recruitment of inflammatory cells leading to an acute immune response with graft rejection in clinical transplantation. Expression and release of many inflammatory cytokines and chemokines, including MCP-1 is regulated by the nuclear factor (NF)-kappaB pathway. Curcumin is an NF-kappaB inhibitor with a variety of biological activities anti-inflammatory, antitumor, antioxidant, and antichemotactic effects. The aim of this study was to examine the effect of curcumin on in vitro MCP-1 release from pancreatic islets.
Methods:
Mouse pancreatic islets in 18-hour cultures were treated with 0 or 10 or 20 micromol/L curcumin and with LPS for an additional 24 hours. MCP-1 levels in culture supernates of islets with versus without curcumin treatment were measured by an ELISA assay.
Results:
We observed that curcumin at the concentration of 20 micromol/L significantly decreased MCP-1 release from mouse islets compared to the control group (P = .005). In addition at both of 10 micromol/L and 20 micromol/L curcumin concentrations there was a decreased level of MCP-1 released from LPS-treated versus control islets (P = .01).
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