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Published on: September 7, 2018
One-step immunochromatography assay for detection of high-level canine serum trypsin-like immunoreactivity
Takaki Waritani1, Masaaki Onda, Yoko Okuno
1Animal Health Products and Chemicals Division, Animal Health Products and Chemicals Research Section, Daiichi Fine Chemical Co., Ltd, Takaoka-shi, Toyama, Japan.
Insights
A new rapid assay kit accurately measures canine trypsin-like immunoreactivity (cTLI) for diagnosing pancreatitis in dogs at the bedside. This test provides critical information for pancreatitis evaluation within minutes.
Area of Science:
- Veterinary Diagnostics
- Biochemical Assays
Background:
- Canine pancreatitis diagnosis often requires specialized laboratory tests.
- A rapid, point-of-care test for canine pancreatitis is needed for timely clinical decisions.
Purpose of the Study:
- To develop and validate a one-step immunochromatography assay kit for measuring canine trypsin-like immunoreactivity (cTLI).
- To enable rapid, bedside estimation of canine pancreatitis.
Main Methods:
- Development of a one-step immunochromatography assay kit for serum cTLI measurement.
- Visual comparison of color strengths for quantitative estimation within 10 minutes.
- Correlation of results with established canine TLI-ELISA methods.
Main Results:
- The assay provides cTLI levels within 10 minutes.
- Results correlate well with canine TLI-ELISA.
- Categorization of cTLI levels: >60 ng/ml (positive), 20-60 ng/ml (weakly positive), <20 ng/ml (negative).
- In a study of 12 dogs, 4 were positive, 2 weakly positive, and 6 negative for pancreatitis.
Conclusions:
- The developed assay kit is effective for rapid, bedside measurement of high cTLI levels.
- Positive results offer critical information for canine pancreatitis evaluation.
- This rapid test facilitates prompt diagnosis and management of pancreatitis in dogs.
Abstract:
We developed a one-step immunochromatography assay kit to measure high levels of canine trypsin-like immunoreactivity (cTLI) for bedside estimation of canine pancreatitis. The serum cTLI level can be determined within 10 min by visual comparison of color strengths in the test and reference zones. The serum cTLI levels determined by this method correlate well with canine TLI-ELISA and can be classified into 3 categories: cTLI levels higher than 60 ng/ml were considered positive; 20-60 ng/ml, weakly positive; and less than 20 ng/ml, negative. Twelve dogs suspected of pancreatitis were examined using this method; 4 dogs were positive, 2 were weakly positive, and 6 were negative. This test can detect a high level of serum cTLI and a positive result in the TLIH test will provide critical information for evaluation of pancreatitis in dogs.
