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Isolation of Murine Lymph Node Stromal Cells
05:47

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Published on: August 19, 2014

CD248/Endosialin is dynamically expressed on a subset of stromal cells during lymphoid tissue development, splenic

Siân Lax1, Tie Zheng Hou, Eric Jenkinson

  • 1Division of Immunity and Infection, Institute for Biomedical Research, MRC Centre for Immune Regulation, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.

FEBS Letters
|July 14, 2007
PubMed

Insights

CD248 (Endosialin) is a novel stromal marker identifying specific lymphoid tissue cells. Its expression changes during development and inflammation, suggesting a role in tissue remodeling.

Area of Science:

  • Immunology
  • Cell Biology
  • Histology

Background:

  • Stromal cell populations in lymphoid tissues (LT) are crucial for immune responses but lack specific markers.
  • Understanding stromal cell heterogeneity is vital for studying LT development, function, and disease.

Purpose of the Study:

  • To identify and characterize a novel stromal cell marker in lymphoid tissues.
  • To investigate the expression patterns of CD248 (Endosialin/TEM1) in different LT compartments and conditions.

Main Methods:

  • Immunohistochemistry and flow cytometry to detect CD248 expression.
  • Analysis of CD248 in developing and adult lymphoid tissues (thymus, lymph node, spleen).
  • Evaluation of CD248 re-expression in a Salmonella-induced splenic enlargement model.

Main Results:

  • CD248 is identified as a differential stromal marker on fibroblasts and pericytes in the thymus, lymph node, and spleen.
  • CD248 expression is high during LT development and significantly decreases in adulthood.
  • CD248 is re-expressed during Salmonella-induced splenic enlargement, correlating with tissue changes.

Conclusions:

  • CD248 defines a specific subset of lymphoid tissue stromal cells.
  • These CD248+ stromal cells are involved in tissue remodeling during LT development, infection, and repair.

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