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Updated: Jul 13, 2026
Immunophenotyping of Orthotopic Homograft (Syngeneic) of Murine Primary KPC Pancreatic Ductal Adenocarcinoma by Flow Cytometry
Published on: October 9, 2018
Immunophenotyping--a tool for the differential diagnostics of lymphadenomegaly
Zuzana Pikalova1, Tomas Szotkowski, Karel Indrak
1Department of Haemato-oncology, University Hospital, Palacky University, Olomouc, Czech Republic.
Insights
Flow cytometry, a method for cell analysis, effectively identified leukemic cell infiltration in lymph nodes. This technique aids in diagnosing lymph node enlargement, particularly in patients with a history of acute lymphoblastic leukemia.
Area of Science:
- Biotechnology
- Immunology
- Cell Biology
Background:
- Flow cytometry enables automated quantification of cellular parameters.
- It can analyze cells from solid tissues after dissociation.
- Immunophenotyping is a key application of flow cytometry.
Observation:
- Lymph node cells were analyzed using immunophenotyping.
- Labeled monoclonal antibodies were employed in the analysis.
Findings:
- Flow cytometry detected leukemic cell infiltration in an extirpated lymph node.
- The study demonstrated the utility of flow cytometry in lymph node assessment.
Implications:
- Flow cytometry offers an efficient method for lymph node evaluation.
- Immunophenotyping contributes to the differential diagnosis of lymph node enlargement.
- This technique is valuable for patients with a history of acute lymphoblastic leukemia.
Background:
Flow cytometry is a method that enables the automated quantification of a set of parameters for a large number of cells or cell-like particles. It is also possible to analyze solid tissues after reduction to a single cell suspension. One of the applications of fl ow cytometry is immunophenotyping.
Aim:
The authors try to introduce the basic physical and biological principles of fl ow-cytometry to the broader public and to prove the benefits of method in lymph nodes assessment.
Method:
A common method of immunophenotyping with the help of labeled monoclonal antibodies applied to lymph node cells was used.
Results:
Flow cytometry revealed the infiltration of the extirpated lymph node with leukemic cells.
Conclusions:
The authors demonstrate fl ow cytometry as a relatively less common but efficient way of lymph node assessment in a patient with an anamnesis of acute lymphoblastic leukemia. They also discuss the contribution of immunophenotyping to the process of the differential diagnostics of lymph nodes enlargement.
